Genes encoding superoxide dismutase (SOD: EC were cloned from NCTC 11351 and UA585 by heterologous complementation of a SOD-deficient mutant. Deletion analysis of the cloned DNA assigned the sod gene to a 1·2 kb insert and this contained an open reading frame of 660 bp. The deduced gene product of 220 amino acids was 71% identical to the iron-containing SOD and 60% identical to the manganese-containing SOD. The recombinant SOD was expressed at high levels in and protected a double mutant from the toxic effects of methyl viologen. Nucleotide sequence analysis of the corresponding gene from showed it to be 92% identical to that from .


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