1887

Abstract

A 2-deoxy--glucose-resistant mutant of a pLZ15-cured derivative of ATCC 393 was isolated on agar medium containing 10 mM 2-deoxy--glucose and 5 g lactose 1 The mutant was impaired in the main glucose transport mechanism, a PTS-type system. Additionally a proton-motive-force-dependent glucose permease was detected. The growth response and the sugar consumption rates of the wild-type and the PTS-deficient mutant suggested that the mutated element of the complex IIABC was, in the wild-type, responsible for a strong repression by glucose and mannose of the lactose and ribose assimilation genes, while assimilation of galactose was only weakly repressed. It is postulated that they are regulated by a different mechanism of catabolite repression.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-5-1141
1994-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/5/mic-140-5-1141.html?itemId=/content/journal/micro/10.1099/13500872-140-5-1141&mimeType=html&fmt=ahah

References

  1. Abe K., Uchida K. Correlation between depression of catabolite control of xylose metabolism and a defect in the phosphoenolpyruvate : mannose phosphotransferase system in Pediococcus halophilus. J Bacteriol 1711793–1800
    [Google Scholar]
  2. Bergmeyer H.U. Methods of Enumatic A4nabsis 3rd edn 1984; 1 Weinheim: Verlag Chemie;
    [Google Scholar]
  3. Bitoun R., de Reuse H., Tonati-Schwartz D., Danchin A. The phosphoenolpyruvate dependent carbohydrate phos-photransferase system of Escherichia coli. Cloning of the ptsYW err region and studies with a pts-lac operon fusion. FEMS Microbiol Lett 1983; 16:163–167
    [Google Scholar]
  4. Chassy B.M., Thompson J. Regulation of lactose-phosphoenolpyruvate-dependent phosphotransferase system and ²-D-phosphogalactosidase galactohydrolase activities in Lactobacillus casei. J Bacteriol 1983a; 154:1195–1203
    [Google Scholar]
  5. Chassy B.M., Thompson J. Regulation and characterization of the galactose phosphoenolpyruvate-dependent-phospho-transferase system in Lactobacillus casei. J Bacteriol 1983b; 154:1204–1214
    [Google Scholar]
  6. Erni B., Zanolari B. Glucose permease of the bacterial phosphotransferase system. J Biol Chem 1986; 261:16398–16403
    [Google Scholar]
  7. Erni B., Zanolari B., Kocher H.P. The mannose permease of Escherichia coli consists of three different proteins. J Biol Chem 1987; 262:5238–5247
    [Google Scholar]
  8. Gonzy-Treboul G., de Waard J.H., Zagorec H., Postma P.W. The glucose permease of the phosphotransferase system of Bacillus sub tills: evidence for IFlc and IIFlc domains. Mol Microbiol 1991; 5:1241–1249
    [Google Scholar]
  9. Jayne-Williams D.J. The application of miniaturized methods for the characterization of various organisms isolated from the animal gut. J Appl Bacteriol 1976; 40:189–200
    [Google Scholar]
  10. Lengeler J., Aulurger A.M., Mayer R., Percher A. The phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system enzyme II as chemoreceptors in chemotaxis of Escherichia coli K-12. Mol and Gen Genet 1981; 183:163–170
    [Google Scholar]
  11. Mason P.W., Carbone D.P., Cushman R.A., Waggoner A.S. The importance of inorganic phosphate in regulation of energy metabolism of Streptococcus lactis. J Biol Chem 1981; 256:1861–1866
    [Google Scholar]
  12. Meadow N.D., Fox D.K., Roseman S. The bacterial phosphoenolpyruvate:glucose phosphotransferase system. Annu Rev Biochem 1990; 59:497–542
    [Google Scholar]
  13. Monod J. Recherches sur la croissance des cultures bacte'riennes 1942 Paris: PhD thesis, Hermanet Cie;
    [Google Scholar]
  14. Postma P.W., Lengeler J.W., Jacobson G.R. Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria. Microbiol Rev 1993; 57:543–594
    [Google Scholar]
  15. Reizer J., Oeutscher J., Sutrina S., Thompson J., Saier M.H. Jr Sugar accumulation in Gram-positive bacteria: exclusion and expulsion mechanisms. Trends Biochem Sci 1985; 10:32–35
    [Google Scholar]
  16. Reizer J., Peterkofsky A., Romano A.H. Evidence for the presence of heat-stable protein (HPr) and ATP-dependent HPr kinase in heterofermentative lactobacilli lacking phosphoenol-pyruvatc :glycose phosphotransferase system. Proc Natl Acad Sci USA 1988; 85:2041–2045
    [Google Scholar]
  17. Romano A., Trifone J.D., Brustoion M. Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in fermentative bacteria. J Bacteriol 1979; 139:93–97
    [Google Scholar]
  18. Saier M.H. Jr, Reizer J. Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Bacteriol 1992; 174:1433–1438
    [Google Scholar]
  19. Strobel H.J. Evidence for catabolite inhibition in the regulation of pentose utilization and transport in the ruminal bacterium Selenomonas ruminantium. Appl Environ Microbiol 1993; 175:40–46
    [Google Scholar]
  20. Tanguey M., Priest F.G., Mitchell W.J. Two glucose transport systems in Bacillus licheniformis. J Bacteriol 1993; 175:2137–2142
    [Google Scholar]
  21. Thompson J., Chassy B.M. Intracellular phosphorylation of glucose analogs via the phosphoenolpyruvate: mannose phosphotransferase systems in Streptococcus lactis. J Bacteriol 1985; 162:224–234
    [Google Scholar]
  22. Thompson J., Saier M.H. Jr Regulation of methyl-²-D-thiogalactopyranoside-6-phosphate accumulation in Streptococcus lactis by exclusion and expulsion mechanism. J Bacteriol 1981; 146:885–894
    [Google Scholar]
  23. Van Rooijen R.J., Dechering K.J., Niek C., Wilmink J., de Vos W.M. Lysines 72, 80 and 213 and aspartic acid 210 of the Lactococcus lactis Lac R repressor are involved in the response to the inducer tagatose-6-phosphate leading to lac operon expression. Protein Eng 1993; 6:201–206
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-5-1141
Loading
/content/journal/micro/10.1099/13500872-140-5-1141
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error