1887

Abstract

By differential hybridization we have identified cDNA clones that are derived from iron-regulated genes in . Sequencing of seven cDNA clones revealed that five clones correspond to encoding triosephosphate isomerase (Tpi1p) and one corresponds to encoding glyceraldehyde-3-phosphate dehydrogenase (Tdh3p). During iron-limited growth mRNA levels for Tpi1p and Tdh3p were at least 3-fold lower than during iron-saturated growth; as shown with a mutant strain this regulation does not require haem synthesis. mRNA half-lives of were 11·5 min (18 min) in low-iron medium and 30 min (32·5 min) in Nigh-iron medium, indicating iron-regulation of transcript half-lives; the stabilities of the and transcripts were not influenced by iron. Increased glycerol production during growth in low-iron, as compared to high-iron medium, is consistent with a modification of the glycolytic flux during iron-limited growth in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-5-1079
1994-05-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/5/mic-140-5-1079.html?itemId=/content/journal/micro/10.1099/13500872-140-5-1079&mimeType=html&fmt=ahah

References

  1. Alber T., Kawasaki G. 1982; Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae.. J Mol Appl Genet 1:419–434
    [Google Scholar]
  2. Braun V., Gunter K., Hantke K. 1991; Transport of iron across the outer membrane. Biol Met I 4:14–22
    [Google Scholar]
  3. Constable A., Quick S., Gray N. K., Hentze M. W. 1992; Modulation of the RNA-binding activity of a regulatory protein by iron in vitro:, switching between enzymatic and genetic function?. Proc Natl Acad Sci USA 89:4554–4558
    [Google Scholar]
  4. Daar I.O., Maquat L. E. 1988; Premature translation termination mediates triosephosphate isomerase mRNA degradation. Mol Cell Biol 8:802–813
    [Google Scholar]
  5. Dancis A., Roman D. G., Anderson G. J., Hinnebusch A. G., Klausner R. D. 1992; Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci USA 89:3869–3873
    [Google Scholar]
  6. Eide D., Guarente L. 1992; Increased dosage of a transcriptional activator gene enhances iron-limited growth of Saccharomyces cerevisiae.. J Gen Microbiol 138:347–354
    [Google Scholar]
  7. Gallwitz D., Sures I. 1980; Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.. Proc Natl Acad Sci USA 77:2546–2550
    [Google Scholar]
  8. Guarente L., Mason T. 1983; Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.. Cell 32:1279–1286
    [Google Scholar]
  9. Hentze M. 1991; Determinants and regulation of cytoplasmic mRNA stability in eukaryotic cells. Biochim Biophys Acta 1090:281–292
    [Google Scholar]
  10. Holland J.P., Holland M. J. 1979; The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.. J Biol Chem 254:9839–9845
    [Google Scholar]
  11. Jones E. W. 1991; Tackling the protease problem in Saccharomyces cerevisiae.. Methods Ensymol 194:428–453
    [Google Scholar]
  12. Lesuisse E., Labbe P. 1989; Reductive and non-reductive mechanisms of iron assimilation by the yeast Saccharomyces cerevisiae.. J Gen Microbiol 135:257–263
    [Google Scholar]
  13. Lesuisse E., Raguzzi F., Crichton R. R. 1987; Iron uptake by the yeast Saccharomyces cerevisiae: involvement of a reduction step. J Gen Microbiol 133:3229–3236
    [Google Scholar]
  14. Lombardo A., Cereghino G. P., Scheffler I. E. 1992; Control of mRNA turnover as a mechanism of glucose repression in Sac-charomyces cerevisiae.. Mol Cell Biol 12:2941–2948
    [Google Scholar]
  15. McAlister L., Holland M. J. 1985; Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3- phosphate dehydrogenase genes. J Biol Chem 260:15013–15018
    [Google Scholar]
  16. Matner R.R., Sherman F. 1982; Differential accumulation of two apo-iso-cytochromes c in processing mutants of yeast. J Biol Chem 257:9811–9821
    [Google Scholar]
  17. Moore P. A., Sagliocco F. A., Wood R. M. C., Brown A. J. P. 1991; Yeast glycolytic mRNAs are differentially regulated. Mol Cell Biol 11:5330–5337
    [Google Scholar]
  18. Nicholas D.J.D. 1957; Microbiological methods for determining magnesium, iron, copper, zinc, manganese, and molybdenum. Methods Encymol 3:1035–1041
    [Google Scholar]
  19. Nonet M., Scafe C., Sexton J., Young R. 1987; Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.. Mol Cell Bioll1602–1611
    [Google Scholar]
  20. Owen D., Kuhn L. C. 1987; Noncoding 3' sequences of the transferrin receptor gene are required for mRNA regulation by iron. EMBO J 6:1287–1293
    [Google Scholar]
  21. Parker R., Herrick D., Peltz S.W., Jacobson A. 1991; Measurement of mRNA decay rates in Saccharomyces cerevisiae.. Methods Emymol 194:415–423
    [Google Scholar]
  22. Philippsen P., Thomas M., Kramer R. A., Davis R. W. 1978; Unique arrangement of coding sequences for 5S5-8S, 18S and 25S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis. J Mol Biol 123:387–404
    [Google Scholar]
  23. Safer B. 1983; 2B or not 2B: regulation of the catalytic utilization of eIF-2. Cell 33:7–8
    [Google Scholar]
  24. Sambrook J., Fritsch T., Maniatis T. 1989 Molecular Cloning. A Eaboratory Manual, 2 nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Sherman F., Fink G. R., Hicks J. B. 1986 Methods in Yeast Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Trumpower B. L., Schmitt M. E., Brown T. A. 1990; A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.. Nucleic Acids Rm 18:3091–3092
    [Google Scholar]
  27. Winkelmann G., van der Helm D., Neilands J. B. , editors. 1987 Iron Transport in Microbes, Plants and Animals. New York: VCH Verlagsgesellschaft;
    [Google Scholar]
  28. Zitomer R.S., Lowry C. V. 1992; Regulation of gene expression by oxygen in Saccharomyces cerevisiae.. Microbiol Rev 56:1–11
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-5-1079
Loading
/content/journal/micro/10.1099/13500872-140-5-1079
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error