1887

Abstract

By differential hybridization we have identified cDNA clones that are derived from iron-regulated genes in Sequencing of seven cDNA clones revealed that five clones correspond to encoding triosephosphate isomerase (Tpi1p) and one corresponds to encoding glyceraldehyde-3-phosphate dehydrogenase (Tdh3p). During iron-limited growth mRNA levels for Tpi1p and Tdh3p were at least 3-fold lower than during iron-saturated growth; as shown with a mutant strain this regulation does not require haem synthesis. mRNA half-lives of were 11·5 min (18 min) in low-iron medium and 30 min (32·5 min) in Nigh-iron medium, indicating iron-regulation of transcript half-lives; the stabilities of the and transcripts were not influenced by iron. Increased glycerol production during growth in low-iron, as compared to high-iron medium, is consistent with a modification of the glycolytic flux during iron-limited growth in

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/content/journal/micro/10.1099/13500872-140-5-1079
1994-05-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-5-1079
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