1887

Abstract

Summary: Mitochondria from spores, anaerobic yeasts and aerobic mycelium of were isolated and the components of the respiratory pathways were analysed. Our data suggest the presence in these mitochondria of three different NADH dehydrogenases, three and two type cytochromes, plus three different terminal oxidases: cytochrome oxidase, an incompletely characterized cytochrome which, by its spectral characteristics, behaves as a cytochrome , and a salicylhydroxamic-acid-sensitive oxidase. The composition of the respiratory chain, as well as the relative proportions of cytochromes and oxidases in the mitochondria, were distinct at each developmental stage. Our data suggest that the development of the respiratory pathways during differentiation is mainly regulated by the oxygen tension. Besides this role, oxygen also influences the relative number of mitochondria in the cells and their ultrastructural organization.

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1994-02-01
2022-01-24
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References

  1. Bartnicki-Garcia S. 1963; Symposium on biochemical bases of morphogenesis in fungi. Bacteriol Rev 27 293 304
    [Google Scholar]
  2. Bartnicki-Garcia S., Nickerson W. J. 1962a; Nutrition, growth and morphogenesis of Mucor rouxii . J Bacteriol 84 829 840
    [Google Scholar]
  3. Bartnicki-Garcia S., Nickerson W. J. 1962b; Assimilation of carbon dioxide and morphogenesis of Mucor rouxii . Biochim Biophys Acta 64 548 551
    [Google Scholar]
  4. Bartnicki-Garcia S., Nelson N., Cota-Robles E. 1968; Electron microscopy of spore germination and cell wall formation in Mucor rouxii . Arch Mikrohiol 63 242 255
    [Google Scholar]
  5. Bendall D. S., Bonner W. D. Jr 1971; Cyanide-insensitive respiration in plant mitochondria. Plant Physiol 47 236 245
    [Google Scholar]
  6. Berry E. A., Trumpower B. L. 1987; Simultaneous determination of heme a, b, and c from pyridine hemochrome spectra. Anal Biochem 161 1 15
    [Google Scholar]
  7. Bertrand H., Argan C. A., Szakacs N. A. 1983; Genetic control of the biogenesis of cyanide-insensitive respiration in Neurospora crassa. . In Mitochondria pp. 495 507 Edited by Schwegen R. J., Wolf K., Kaudewits F. Berlin: W. De-Gruyter;
    [Google Scholar]
  8. Borgia P. T., Gokul N. K., Phillips G. J. 1985; Respiratory-competent conditional developmental mutant of Mucor racemosus. J Bacteriol 164 1049 1056
    [Google Scholar]
  9. Bruinenberg P. M., Van-Dijken J. P., Kuenen J. G., Scheffers W. A. 1985; Oxidation of NADH and NADPH by mitochondria from the yeast Candida utilis . J Gen Microbiol 131 1043 1051
    [Google Scholar]
  10. Cano-Canchola C., Escamilla E., Ruiz-Herrera J. 1988; Envionmental control of the respiratory system in the dimorphic fungus Mucor rouxii . Gen Microbiol 134 2993 3000
    [Google Scholar]
  11. Hatefi Y. 1985; The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem 54 1015 1069
    [Google Scholar]
  12. Henry M. F., Nyns E. J. 1975; Cyanide-insensitive respiration. An alternative mitochondrial pathway. Subcell Biochem 4 1 65
    [Google Scholar]
  13. Lambers H. 1980; The physiological significance of cyanide-resistant respiration in higher plants. Plant Cell Environ 3 293 302
    [Google Scholar]
  14. Lambers H. 1982; Cyanide-resistant respiration: a nonphos-phorylating electron pathway acting as an energy overflow. Physiol Plant 55 478 485
    [Google Scholar]
  15. Lee C. P., Ernster L. 1967; Energy-coupling in nonphos-phorylating submitochondrial particles. Methods Enzymol 10 543 548
    [Google Scholar]
  16. Lehninger A. L. 1964 The Mitochondrion New York & Amsterdam: Benjamin;
    [Google Scholar]
  17. Rogers P. J., Clark-Walker G. D., Stewart P. R. 1974; Effects of oxygen and glucose on energy metabolism and dimorphism of Mucorgenevensis grown in continuous culture: reversibility of yeast-mycelium conversion. J Bacteriol 119 282 293
    [Google Scholar]
  18. Ruiz-Herrera J., Ruiz A, Lopez-Romero E. 1983; Isolation and biochemical analysis of Mucor bacilliformis monomorphic mutants. J Bacteriol 156 264 272
    [Google Scholar]
  19. Salcedo-Hernandez R., Ruiz-Herrera J. 1993; Isolation and characterization of a mycelial cytochrome aa3 deficient mutant, and the role of mitochondria in dimorphism of Mucor rouxii . Exp Mycol 17 142 154
    [Google Scholar]
  20. Schwitzguebel J., Palmer J. M. 1982; Properties of mitochondria as a function of growth stages of Neurospora crassa . J Bacteriol 149 612 619
    [Google Scholar]
  21. Shepherd M. G., Chin C. M., Sullivan P. A. 1978; The alternate respiratory pathway of Candida albicans . Arch Microbiol 116 61 67
    [Google Scholar]
  22. Siedow J. N., Berthold D. A. 1986; The alternative oxidase: a cyanide resistant respiratory pathway in higher plants. Physiol Plant 66 569 573
    [Google Scholar]
  23. Storck R., Morrill R. C. 1971; Respiratory-deficient, yeast-like mutant of Mucor. Biochem Genet 5 467 479
    [Google Scholar]
  24. Zitomer R. S., Lowry C. V. 1992; Regulation of gene expression by oxygen in Saccharomyces cerevisiae. Microbiol Rev 56 1 11
    [Google Scholar]
  25. Zorzopulos J., Jobbagy A. J., Terenzi H. F. 1973; Effects of ethylenediaminetetraacetate and chloramphenicol in Mucor rouxii. J Bacteriol 115 1198 1204
    [Google Scholar]
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