Cloning and sequencing of encoding sakacin P, the bacteriocin produced by LTH 673 Free

Abstract

Summary: Sakacin P is a heat-stable, unmodified peptide bacteriocin produced by LTH 673. The strain was isolated from fermented dry sausages and is well adapted to this habitat. The bacteriocin inhibits the growth of the opportunistic food pathogens and and therefore, it may improve the hygienic status of fermented food, i.e. meat products. Oligonucleotide probes were designed from the N-terminal amino acid sequence of sakacin P and used to identify , the structural gene of sakacin P, on the chromosome of LTH 673. was cloned into NM554 and the nucleotide sequence of the gene and its adjacent regions were determined. Sakacin P appears to be synthesized as a prepeptide of 61 amino acids which is proteolytically processed to the mature bacteriocin consisting of 43 amino acids. Sequencing of the cloned fragment also revealed the presence of two other open reading frames and , which are located upstream and downstream of , respectively, putatively encoding proteins of 52 and 98 amino acids, respectively. The functions of both ORFs remain unknown. Primer extension analysis revealed a promoter upstream of . Two transcripts of approximately 0.35 and 1.0 kb were detected by Northern hybridization encoding either only , or both and , respectively.

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1994-02-01
2024-03-29
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References

  1. Barefoot S. F., Klaenhammer T. R. 1983; Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol 45:1808–1815
    [Google Scholar]
  2. van Belkum M. J., Hayema B. J., Geis A., Kok J., Venema G. 1989; Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid. Appl Environ Microbiol 55:1187–1191
    [Google Scholar]
  3. van Belkum M. J., Hayema B. J., Jeeniga R. F., Kok J., Venema G. 1991; Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ Microbiol 57:492–498
    [Google Scholar]
  4. van Belkum M. J., Kok J., Venema G. 1992; Cloning, sequencing, and expression in Escherichia coli of IcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6. Appl Environ Microbiol 58:572–577
    [Google Scholar]
  5. Buchman G. W., Banerjee S., Hansen J. N. 1988; Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic. J Biol Chem 263:16260–16266
    [Google Scholar]
  6. Daeschel M. A. 1989; Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technol 43:164–167
    [Google Scholar]
  7. Daeschel M. A., Klaenhammer T. R. 1985; Association of a 13'6 megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl Environ Microbiol 50:1538–1541
    [Google Scholar]
  8. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. 1981; Recovery of DNA fragments from agarose gels using DEAE- paper. Anal Biochew 112:295
    [Google Scholar]
  9. Foegeding P. M., Thomas A. B., Pilkington D. H., Klaen¬hammer R. 1992; Enhanced control of Listeria monocytogenes by in /////-produced pediocin during dry fermented sausage production. Appl Environ Microbiol 58:884–890
    [Google Scholar]
  10. Gierasch L. M. 1989; Signal sequences. Biochemistry 28:923–930
    [Google Scholar]
  11. Grinstead D. A., Barefoot S. F. 1992; Jenseniin G, a heat-stable bacteriocin produced by Propionibacterium jensenii PI 26. Appl Environ Microbiol 58:215–220
    [Google Scholar]
  12. Hammes W. P., Bantleon A., Min S. 1990; Lactic acid bacteria in meat fermentation. FEMS Microbiol Rev 87:165–174
    [Google Scholar]
  13. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
    [Google Scholar]
  14. Hastings J. W., Sailer M., Johnson K., Roy K. L., Vederas J. C., Stiles M. 1991; Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol 173:7491–7500
    [Google Scholar]
  15. Hgchard Y., Derijard B., Letellier F., Cenatiempo Y. 1992; Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J Gen Microbiol 138:2725–2731 von Heijne G. 1988; Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta 947:307–333
    [Google Scholar]
  16. Hoick A., Axelsson S., Birkeland S. E., Aukrust T., Blom H. 1992; Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb 706. J Gen Microbiol 138:2715–2720
    [Google Scholar]
  17. Holo H., Nilssen O., Nes I. F. 1991; Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris\ isolation and characterisation of the protein and its gene. J Bacteriol 173:3879–3887
    [Google Scholar]
  18. Jim£nez-Dfaz R., Rios-Snchez R. M., Desmazeaud M., Ruiz-Barba J. L., Piard J.-C. 1993; Plantaricin S and T, two new bacteriocins produced by Lactobacillus plantarum LPCOIO isolated from a green olive fermentation. Appl Environ Microbiol 59:1416–1424
    [Google Scholar]
  19. Joerger M. C., Klaenhammer T. R. 1986; Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol 167:439–446
    [Google Scholar]
  20. Joerger M. C., Klaenhammer T. R. 1990; Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J. J Bacteriol 172:6339–6347
    [Google Scholar]
  21. Klaenhammer T. R. 1988; Bacteriocins of lactic acid bacteria. Biochimie 70:337–349
    [Google Scholar]
  22. Lozano J. C. N., Nissen-Meyer J., Sletten K., Pélaz C., c Nes I. F. 1992; Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici. J Gen Microbiol 138:1985–1990
    [Google Scholar]
  23. de Man J. C., Rogosa M., Sharpe M. E. 1960; A method for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
    [Google Scholar]
  24. Marugg J. D., Gonzalez C. F., Kunka B. S., Ledeboer A. M., Pucci M. J., Toonen M. Y., Walker S. A., Zoetmulder M. J., Vandenbergh P. A. 1992; Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, a bacteriocin produced from Pediococcus acidilactici PAC1.0. Appl Environ Microbiol 58:2360–2367
    [Google Scholar]
  25. Mertvedt C. I., Nes I. F. 1990; Plasmid-associated bacteriocin production by a Lactobacillus sake L45. J Gen Microbiol 136:1601–1607
    [Google Scholar]
  26. Muriana P. M., Klaenhammer T. R. 1991; Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp. J Bacteriol 173:1779–1788
    [Google Scholar]
  27. Obst M. 1993; Genetische und physiologische Untersuchungen des Laktosemetabolismus von Lactobacillus sake und L. curvatus. PhD thesis Universitat Hohenheim, Germany;
    [Google Scholar]
  28. Pugsley A. 1993; The complete general secretory pathway in Gram-negative bacteria. Microbiol Rev 57:50–108
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
    [Google Scholar]
  30. Schillinger, U„ Kaya M., Lucke F. K. 1991; Behaviour of Listeria monocytogenes in meat and its control by a bacteriocin producing strain of Lactobacillus sake. J Appl Bacteriol 70:473–478
    [Google Scholar]
  31. Simonen M., Palva I. 1993; Protein secretion in Bacillus species. Microbiol Rev 57:109–137
    [Google Scholar]
  32. Stoddard G. W., Petzel J. P., van Belkum M. J., Kok J., McKay L. L. 1992; Molecular analyses of the lactococcin A gene cluster from Eactococcus lactis subsp. lactis biovar diacetylactis WM4. Appl Environ Microbiol. 58:1952–1961
    [Google Scholar]
  33. Tagg J. R., Dajani A. S., Wannamaker L. W. 1976; Bacteriocins of Gram-positive bacteria, bacterial Rep. 40:722–756
    [Google Scholar]
  34. Tichaczek P. S., Nissen-Meyer J., Nes I. F., Vogel R. F., Hammes W. P. 1992; Characterization of the bacteriocins cur¬vacin A from Eactobacillus curvatus LTH 1174 and sakacin P from L. sake LTH673. Syst Appl Microbiol 15:460–468
    [Google Scholar]
  35. Tichaczek P. S., Vogel R. F., Hammes W. P. 1993; Cloning and sequencing of cur A encoding curvacin A, the bacteriocin produced bv Lactobacillus curvatus LTH 1174. Arch Microbiol. 160:279–283
    [Google Scholar]
  36. Thomas D. Y., Dubus G., Narang S. 1982; Escherichia coli plasmid vectors containing synthetic translational initiation sequences and ribosome binding sites fused with the lacZ gene. Gene 19:211–219
    [Google Scholar]
  37. Vincent J. G., f Veomett R. C., Riley R. F. 1959; Antibacterial activity associated with Eactobacillus acidophilus. J Bacteriol 78:477–484
    [Google Scholar]
  38. Vogel R. F., Pohle B. S., Tichaczek P. S., Hammes W. P. 1993; The competitive advantage of Lactobacillus curvatus LTH 1174 in sausage fermentations is caused by formation of curvacin A. Syst Appl Microbiol 16:457–462
    [Google Scholar]
  39. Wheater D. M., Hirsch A., Mattick A. T. R. 1951; Lacto- bacillin’, an antibiotic from lactobacilli. Nature 168:659
    [Google Scholar]
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