1887

Abstract

Summary: Yeast cells of produced germ tubes in a salt-glucose medium containing 4% calf serum at pH 7 and 37 °C. Hyphal growth continued for 24 h and the filaments did not revert to yeast cells. When cells were grown at pH 4, reversion to yeast growth was observed, despite the presence of serum. The elongation of hyphae was inhibited within 30 min. The distribution of microtubules and microfilaments during pH-regulated morphological transition was studied by an immunofluorescence technique using an antitubulin antibody with a FITC-conjugated secondary antibody, and by staining with tetramethylrhodaminyl phalloidin for filamentous actin and actin granules. After changing to acidic conditions, microtubules were distributed normally in the cytoplasm; however, microfilaments disappeared from hyphal cells, and actin granules were localized at the site of budding. These results show that microfilaments play an important role during pH-regulated morphological transition.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-2-281
1994-02-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/2/mic-140-2-281.html?itemId=/content/journal/micro/10.1099/13500872-140-2-281&mimeType=html&fmt=ahah

References

  1. Buffo J., Herman M. A., Soli D. R. 1984; A characterization of pH-regulated dimorphism in Candida albicans. Mycopathologia 85 21 30
    [Google Scholar]
  2. Fronk J., Toczko K. 1987; Changes in the phosphorylation of non-histone proteins during differentiation of a lower eukaryote Physarum polycephalum. Biochem Biophys Res Commun 142 188 193
    [Google Scholar]
  3. Gupta P., Prasad R. 1993; Levels of plasma membrane H+- ATPase do not change during growth and morphogenesis of Candida albicans. FEAIX Microbiol Pett 106 165 170
    [Google Scholar]
  4. Kaur S., Mishra P. 1991; Dimorphism-associated changes in plasma membrane H+-ATPase activity of Candida albicans. Arch Microbiol 156 412 415
    [Google Scholar]
  5. Kaur S., Mishra P., Prasad R. 1988; Dimorphism-associated changes in intracellular pH of Candida albicans. Biochim Biophys Acta 972 277 282
    [Google Scholar]
  6. Lee K.L., Buckley H. R., Campbell C. C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 13 148 153
    [Google Scholar]
  7. Lydan M. A., O’Day D. H. 1988; Differential developmental functions for calmodulin in Dictyosteliunr. trifluoperazine and R24571 both inhibit cell and pronuclear fusion but enhance gamete formation. Exp Cell Res 178 51 63
    [Google Scholar]
  8. Muthukumar G., Nickerson K. W. 1984; Ca(II)-calmodulin regulation of fungal dimorphism in Ceratocystis ulmi. J Bacterial 159 390 392
    [Google Scholar]
  9. Muthukumar G., Nickerson A. W., Nickerson K. W. 1987; Calmodulin levels in yeasts and filamentous fungi. FEMS Microbiol Lett 41 253 255
    [Google Scholar]
  10. Orlowski M., Sypherd P. S. 1978; Regulation of translation rate during morphogenesis in fungus Mucor. Biochemistry 17 560 574
    [Google Scholar]
  11. Paranjape V., Gupta Roy B., Datta A. 1990; Involvement of calcium, calmodulin and protein phosphorylation in morphogenesis of Candida albicans. J Gen Microbiol 136 2141 2154
    [Google Scholar]
  12. Pollack J. H., Hashimoto T. 1987; The role of glucose in the pH regulation of germ-tube formation in Candida albicans. J Gen Microbiol 133 415 424
    [Google Scholar]
  13. St Leger R., Roberts D. W., Staples R. C. 1989; Calcium and calmodulin-mediated protein synthesis and protein phosphorylation during germination, growth and protease production by Metarhizium anisopliae. J Gen Microbiol 135 2141 2154
    [Google Scholar]
  14. Sinclair J. H., Rickwood D. 1985; Major changes in phosphorylation of chromatin associated non-histone proteins accompany development in slime mold Dictyostelium discoideum. Biochem J 229 771 778
    [Google Scholar]
  15. Stewart E., Gow N. A. R., Bowen D. V. 1988; Cytoplasmic alkalinization during germ tube formation in Candida albicans. J Gen Microbiol 134 1079 1087
    [Google Scholar]
  16. Stewart E., Hawser S., Gow N. A. R. 1989; Changes in internal and external pH accompanying growth of Candida albicans: studies of non-dimorphic variants. Arch Microbiol 151 149 153
    [Google Scholar]
  17. Uyeda T. Q. P., Furya M. 1986; Effects of low temperature and calcium on microfilament structure in flagellates of Physarum polycephalum. Exp Cell Res 165 461 472
    [Google Scholar]
  18. Yokoyama K., Takeo K. 1983; Differences of asymmetrical division between the pseudomycelial and yeast forms of Candida albicans and their effect on multiplication. Arch Microbiol 134 251 253
    [Google Scholar]
  19. Yokoyama K., Kaji H., Nishimura K., Miyaji M. 1990; The role of microfilaments and microtubules in apical growth and dimorphism of Candida albicans. J Gen Microbiol 136 1067 1075
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-2-281
Loading
/content/journal/micro/10.1099/13500872-140-2-281
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error