1887

Abstract

Pectin methylesterase (PME) was purified from the supernatant of strain Bd90. SDS-PAGE showed a single band at 42 kDa, but this band corresponded to two distinct isoforms observed by IEF-PAGE at pl values of 7.0 and 7.4. PME was produced during the exponential phase of fungal growth and independently of the carbon source. Unlike other pectinases of , which are polymorphic, no differences were observed between the PME profiles of 25 strains of different origins. Polyclonal antibodies were raised against purified PME from , and immunochemical comparisons with PMEs from and showed the presence of common epitopes between these different enzymes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-12-3249
1994-12-01
2019-11-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-12-3249
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error