%0 Journal Article %A Eastman, Deborah %A Dworkin, Martin %T Endogenous ADP-ribosylation during development of the prokaryote Myxococcus xanthus %D 1994 %J Microbiology, %V 140 %N 11 %P 3167-3176 %@ 1465-2080 %R https://doi.org/10.1099/13500872-140-11-3167 %K myxobacteria %K prokaryotic development %K endogenous ADP-ribosylation %K Myxococcus xanthus %I Microbiology Society, %X We examined endogenous ADP-ribosylation of proteins during the development of the prokaryote Myxococcus xanthus. In vivoand in vitroendogenous ADP-ribosylation of M. xanthusproteins was detected and the profile of modified proteins changed during development. Adenosine and nicotinamide inhibited ADP-ribosylation. Nicotinamide stimulated cells at low density to develop, in a manner similar to that previously observed with adenosine. Higher concentrations of nicotinamide inhibited aggregation. The in vivoeffects of nicotinamide on developing M. xanthuscells correlate with its in vitroeffects on ADP-ribosylation and the developmental profile of putative ADP-ribosylation substrates. These results suggest that ADP-ribosylation may regulate developmental proteins in M. xanthus. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-11-3167