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We examined endogenous ADP-ribosylation of proteins during the development of the prokaryote Myxococcus xanthus. In vivoand in vitroendogenous ADP-ribosylation of M. xanthusproteins was detected and the profile of modified proteins changed during development. Adenosine and nicotinamide inhibited ADP-ribosylation. Nicotinamide stimulated cells at low density to develop, in a manner similar to that previously observed with adenosine. Higher concentrations of nicotinamide inhibited aggregation. The in vivoeffects of nicotinamide on developing M. xanthuscells correlate with its in vitroeffects on ADP-ribosylation and the developmental profile of putative ADP-ribosylation substrates. These results suggest that ADP-ribosylation may regulate developmental proteins in M. xanthus.
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