1887

Abstract

The regulation of glycine betaine accumulation by was investigated. The accumulation of glycine betaine was regulated by the osmotic pressure of the medium and the low affinity transport system played the major role in this regulation. Mutants were isolated that lack the low affinity, osmotically activated glycine betaine/proline transport system. Such mutants accumulated glycine betaine via the high affinity system but the glycine betaine pool was smaller and responded poorly to osmotic pressure changes. The regulation of glycine betaine transport has revealed that at the steady state net influx is reduced and that this is achieved by inhibition of both the low affinity and the high affinity transport systems. Cells pre-loaded with glycine betaine exhibited a reduced for both systems: the low affinity system was reduced in activity fivefold and the high affinity system was reduced 10-fold and became virtually undetectable. Although glycine betaine transport at the steady state is reduced, retention of the compatible solute is an active process since addition of an uncoupler provokes rapid release of the accumulated material. These data suggest that feedback regulation of the activity of the uptake systems is a major mechanism for controlling the level of compatible solute accumulation.

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1994-11-01
2021-08-02
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References

  1. Anderson C.B., Witter L D. 1982; Glutamine and proline accumulation by Staphylococcus aureuswith reduction in water activity. Appl Environ Microbiol 43:1501–1503
    [Google Scholar]
  2. Bae J.-H., Miller K.J. 1992; Identification of two proline transport systems in Staphylococcus aureusand their possible role in osmoregulation. Appl Environ Microbiol 58:471–475
    [Google Scholar]
  3. Bae J.H., Anderson S. H., Miller K. J. 1993; Identification of a high affinity glycine betaine transport in Staphylococcus aureus . Appl Environ Microbiol 59:2734–2736
    [Google Scholar]
  4. Booth I. R., Cairney J., Sutherland L., Higgins C. F. 1988; Enteric bacteria and osmotic stress: an integrated homeostatic. J Appl Bacteriol Symp Suppl 35S–49S
    [Google Scholar]
  5. Csonka L.N. 1989; Physiological and genetic responses of bacteria to osmotic stress.. Microbiol Rev 53:121–147
    [Google Scholar]
  6. Epstein W. 1986; Osmoregulation of potassium transport in Escherichia coli . Fems Microbiol Rev 39:73–78
    [Google Scholar]
  7. Graham J.E., Wilkinson B. J. 1992; Staphylococcus aureusosmoregulation: roles for choline, glycine betaine, proline and taurine. J Bacteriol 174:271 1–2716
    [Google Scholar]
  8. Kaenjak A., Graham J. E., Wilkinson B. J. 1993; Choline transport activity in Staphylococcus aureusinduced by osmotic stress and low phosphate concentrations. J Bacterial 175:2400–2406
    [Google Scholar]
  9. Koo S.P., Higgins C. F., Booth I. R. 1991; Regulation of compatible solute accumulation in Salmonella typhimurium: evidence for a glycine betaine efflux system.. J Gen Microbiol 137:2617–2625
    [Google Scholar]
  10. Koujima I., Hayashi H., Tomochika A., Okaba A., Kanemasa Y. 1978; Adaptational change in proline and water content after alteration of environmental salt concentration. Appl Environ Microbiol 35:467–470
    [Google Scholar]
  11. Meury J., Robin A., Monier-Champeix P. 1985; Turgor-controlled K+fluxes and their pathways in Escherichia coli.. Eur J Biochem 151:613–619
    [Google Scholar]
  12. Miller K. J., Zelt S.C., Bae J.-H. 1991; Glycine betaine and proline are the principal compatible solutes of Staphylococcus aureus.. Curr Microbiol 23:131–137
    [Google Scholar]
  13. Mitchell P., Moyle J. 1956; Osmotic function and structure in bacteria.. Symp Soc Gen Microbiol 6:150–180
    [Google Scholar]
  14. Pattee P.A., Neveln D. S. 1975; Transformation analysis of three linkage groups in Staphylococcus aureus . J Bacteriol 124:201–211
    [Google Scholar]
  15. Pourkomailian B., Booth I. R. 1992; Glycine betaine transport by Staphylococcus aureus:,evidence for two transport systems and for their possible roles in osmoregulation.. J Gen Microbiol 138:2515–2518
    [Google Scholar]
  16. Rhoads D.E., Epstein W. 1978; Cation transport in Escherichia coliIX. Regulation of potassium transport.. J Gen Physiol 72:283–295
    [Google Scholar]
  17. Rowland G. C., Giffard P. M., Booth I. R. 1984; Genetic studies of the phslocus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism and pH homeostasis.. FEBS Lett 173:295–300
    [Google Scholar]
  18. Stimeling K. W., Graham J. E., Kaenjak A., Wilkinson B. J. 1994; Evidence for feedback (trans)regulation of, and two systems for, glycine betaine transport in Staphylococcus aureus. . Microbiology 140:3139–3144
    [Google Scholar]
  19. Townsend D.E., Wilkinson B. J. 1992; Proline transport in Staphylococcus aureus:,a high affinity system and a low affinity system involved in osmoregulation. J Bacteriol 174:2702–2710
    [Google Scholar]
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