1887

Abstract

The gene () encoding carboxynorspermidine decarboxylase (CANS DC), the last enzyme in norspermidine biosynthesis, in was isolated by immuno-screening and its complete nucleotide sequence was determined. Sequence analysis of the subcloned fragment (2.0 kb) revealed an ORF of 1131 bp encoding a protein of 377 amino acids with a calculated molecular mass of 42008 Da. The sequence of 20 N-terminal amino acids of purified CANS DC was found to be identical to that predicted from the gene. A putative ribosome binding sequence was observed 8 bp upstream from the translation start site (ATG), and promoter- and terminator-like sequences were detected upstream and downstream of the ORF, respectively. Database searches identified no similar proteins, but the deduced amino acid sequence contained a putative pyridoxal 5′-phosphate binding region similar to those of the bacterial -2,6-diaminopimelate decarboxylases and eukaryotic ornithine decarboxylases. Another full ORF was found on the opposite strand downstream from the gene. It encoded a protein of 69 amino acids with a calculated molecular mass of 7441 Da, which exhibited some weak similarity to a repressor protein of , in the helix-turn-helix DNA binding domain, but did not appear to be expressed in the host cells.

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1994-11-01
2022-01-17
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References

  1. Andersson S.G.E, Kurland C. G. 1990; Codon preference in free-living microorganisms. Microbiol Rev 54:198–210
    [Google Scholar]
  2. Baumann P., Schubert R. H. W. 1984; Family II. Vibrionaceae Veron 1965, 5245AL . In Bergey’s Manual of Systematic Bacteriology 1: pp. 516–538 Edited by Krieg N. R., Holt J. G. . Baltimore: Williams and Wilkins;
    [Google Scholar]
  3. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Resi 1513–1523
    [Google Scholar]
  4. Blatch G.L., Woods D. R. 1991; Nucleotide sequence and analysis of the Vibrio alginolyticus scrrepressor-encoding gene scr R. Gene 101:45 –50
    [Google Scholar]
  5. Blatch G. L., Scholle R. R., Woods D. R. 1990; Nucleotide sequence and analysis of the Vibrio alginolyticussucrose uptake- encoding region. Gene 95:17–23
    [Google Scholar]
  6. Boyer H.W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli . J Mol Biol 41:459–472
    [Google Scholar]
  7. Boyle S. M., Markham G. D., Hafner E. W., Wright J. M., Tabor H., Tabor C. W. 1984; Expression of the cloned genes encoding the putrescine biosynthetic enzymes and methionine adenosyl- transferase of Escherichia coli(spe A, spe B, spe Cand met K . Gene 30:129–136
    [Google Scholar]
  8. Farrell D. H., Mikesell P., Actis L. A., Crosa J. H. 1990; A regulatory gene, ang R,of the iron uptake system of Vibrio anguillarum: similarity with phage P22 eroand regulation by iron. Gene 86:45–51
    [Google Scholar]
  9. Hamana K., Matsuzaki S. 1992; Polyamines as a chemo- taxonomic marker in bacterial systematics. Crit Rev Microbiol 18:261–283
    [Google Scholar]
  10. Harley D.K., Reynolds R. P. 1987; Analysis of E. colipromoter sequences. Nucleic Acids Res 15:2343–2361
    [Google Scholar]
  11. Helfman D. M., Feramisco J. R., Fiddes J. C., Thomas G. P., Hughes S. H. 1983; Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. Proc Natl Acad Sci USA 80:31–35
    [Google Scholar]
  12. Janda J. M., Powers C., Bryant R. G., Abbott S. L. 1988; Current perspectives on the epidemiology and pathogenesis of clinically significant Vibriospp. Clin Microbiol Rev 1:245–267
    [Google Scholar]
  13. Laemmli U.K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  14. Mills D.A., Flickinger M. C. 1993; Cloning and sequence analysis of the meso-diaminopimelate decarboxylase gene from Bacillus methanolicusMGA3 and comparison to other decarboxylase genes. Appl Environ Microbiol 59:2927–2937
    [Google Scholar]
  15. Moore R.C., Boyle S. M. 1990; Nucleotide sequence and analysis of the spe Agene encoding biosynthetic arginine decarboxylase in Escherichia coli . J Bacteriol 172:4631–4640
    [Google Scholar]
  16. Murray M. G., Thompson W. F. 1980; Rapid isolation of high- molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
    [Google Scholar]
  17. Nakagami S., Matsunaga H., Oka N., Yamane A. 1991; Preparation of enzyme-conjugated DNA probe and application of the universal probe.. Anal Biochem 198:75–79
    [Google Scholar]
  18. Nakao H., Ishii M., Shinoda S., Yamamoto S. 1989; Purification and some properties of a novel L-2,4-diaminobutyric acid decarboxylase from Vibrio alginolyticus . J Gen Microbiol 135:345–351
    [Google Scholar]
  19. Nakao H., Shinoda S., Yamamoto S. 1990; Purification and properties of carboxynorspermidine decarboxylase, a novel enzyme involved in norspermidine biosynthesis, from Vibrio alginolyticus . J Gen Microbiol 136:1699–1704
    [Google Scholar]
  20. Nakao H., Shinoda S., Yamamoto S. 1991; Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in Vibrio alginolyticu . J Gen Microbiol 137:1737–1742
    [Google Scholar]
  21. Norrander J., Kempe T., Messing J. 1983; Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  22. Pabo C.O., Sauer R. T. 1984; Protein-DNA recognition. Annu Rev Biochem 53:293–321
    [Google Scholar]
  23. Platt T. 1986; Transcription termination and regulation of gene expression. Annu Rev Biochem 55:339–372
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual , 2 nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  26. Shine J., Dalgarno L. 1974; The 3'-Terminal sequence of Escherichia coli16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding site. Proc. Natl Acad Sci USA 71:1342–1346
    [Google Scholar]
  27. Smith D. M., Thomas N. R., Gani D. 1991; A comparison of pyridoxal 5'-phosphate dependent decarboxylase and transaminase enzymes at a molecular level. Experientia 47:1104–1118
    [Google Scholar]
  28. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
    [Google Scholar]
  29. Szumanski M.B.W, Boyle S. M. 1990; Analysis and sequence of the spe Bgene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli . J Bacteriol 172:538–547
    [Google Scholar]
  30. Tabor C.W., Tabor H. 1987; The spe Espe Doperon of Escherichia coli: formation and processing of a proenzyme form of S-adenosylmethionine. J Biol Chem 262:16037–16040
    [Google Scholar]
  31. Tait G.H. 1976; A new pathway for the biosynthesis of spermidine. Biochem Soc Trans 4:610–612
    [Google Scholar]
  32. Takeuchi H., Shibano Y., Morihara K., Fukushima J., Inami S., Keil B., Gilles A., Kawamoto S., Okuda K. 1992; Structural gene and complete amino acid sequence of Vibrio alginolyticuscollagenase. Biochem J 281:703–708
    [Google Scholar]
  33. Yamamoto S., Shinoda S., Makita M. 1979; Occurrence of norspermidine in some species of genera Vibrioand Beneckea . Biochem Biophys Res Commun 87:1102–1108
    [Google Scholar]
  34. Yamamoto S., Hamanaka K., Suemoto Y., Ono B., Shinoda S. 1986; Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio . Can J Microbiol 32:99–103
    [Google Scholar]
  35. Yamamoto S., Chowdhury R. M. A., Kuroda M., Nakano T., Koumoto Y., Shinoda S. 1990; Further study on polyamine compositions in Vibrionaceae . Can J Microbiol 37:148–153
    [Google Scholar]
  36. Yamamoto S., Tsuzaki Y., Tougou K., Shinoda S. 1992; Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus . J Gen Microbiol 138:1461–1465
    [Google Scholar]
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