1887

Abstract

A laboratory-scale river microcosm was used to investigate the effect of the anionic surfactant sodium dodecyl sulphate (SDS) on the attachment of five strains to natural river-sediment surfaces. Three of the strains were chosen for their known ability to express alkylsulphatase enzymes capable of hydrolysing SDS, and the other two for their lack of such enzymes. One strain from each category was isolated from the indigenous bacterial population present in the river sediment used; other isolates were from soil or sewage. The alkylsulphatase phenotypes were confirmed by gel zymography of cell extracts. Addition of SDS to mixed suspensions of river sediment with any one of the biodegradation-competent strains stimulated the attachment of bacteria to the sediment particles. In contrast, the attachment of biodegradation-incompetent strains was weak and, moreover, was unaffected by SDS. The SDS-stimulated attachment for competent organisms coincided with rapid biodegradation of the surfactant. The primary intermediate of SDS biodegradation, dodecan-1-ol, accumulated transiently, and the numbers of attached bacteria correlated closely with the amount of dodecan-1-ol present. Direct addition of dodecan-1-ol also stimulated attachment but the effect was more immediate compared with SDS, when there was a lag period of approximately 2 h. To account for these observations, a model is proposed in which SDS stimulates the attachment of biodegradation-competent bacteria through its conversion to dodecan-1-ol, and it is hypothesized that the observed reversibility of the attachment is due to the subsequent removal of dodecan-1-ol by further bacterial metabolism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-11-2999
1994-11-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/11/mic-140-11-2999.html?itemId=/content/journal/micro/10.1099/13500872-140-11-2999&mimeType=html&fmt=ahah

References

  1. Anderson D.J., Day M.J., Russell N.J., White G.F. Die-away kinetic analysis of the capacity of epilithic and planktonic bacteria from clean and polluted river water to biodegrade sodium dodecyl sulphate. Appl Environ Microbiol 1990; 56:758–763
    [Google Scholar]
  2. Aronstein B.N., Alexander M. Surfactants at low concentrations stimulate biodegradation of sorbed hydrocarbons in samples of aquifer sands and soil slurries. Environ Toxicol Chem 1992; 11:1227–1233
    [Google Scholar]
  3. Aronstein B.N., Alexander M. Effect of a non-ionic surfactant added to the soil surface on the biodegradation of aromatic hydrocarbons within soil. Appl Microbiol Biotechnol 1993; 39:386–390
    [Google Scholar]
  4. Aronstein B.N., Calvillo Y.M., Alexander M. Effects of surfactants at low concentrations on the desorption and biodegradation of sorbed aromatic hydrocarbons in soil. Environ Sri technol 1991; 25:1728–1731
    [Google Scholar]
  5. Clarke K.R., Joint I.R. Methodology for estimating numbers of free-living and attached bacteria in estuarine water. Appl Environ Microbiol 1986; 51:1110–1120
    [Google Scholar]
  6. Costerton J.W., Cheng K.-J., Geesey G.G., Ladd T.I., Nickel J.C., Dasgupta M., Marrie T.J. Bacterial biofilms in nature and disease. Annu Rev Microbiol 1987; 41:435–464
    [Google Scholar]
  7. Crescenzi A.M.V. Microbial degradation of short chain alkyl sulphate esters. 1983 PhD Thesis, University of Wales;
    [Google Scholar]
  8. Dodgson K.S., Fitzgerald J.W., Payne W.J. Chemically defined inducers of alkylsulfatases present in Pseudomonas C12B. Biochem J 1974; 138:53–62
    [Google Scholar]
  9. Goldberg S., Konis Y., Rosenberg M. Effect of cetyl pyridinium chloride on microbial adhesion to hexadecane and polystyrene. Appl Environ Microbiol 1990; 56:1678–1682
    [Google Scholar]
  10. Guerin W.F., Boyd S.A. Differential bioavailability of soil-sorbed naphthalene to two bacterial species. Appl Environ Microbiol 1992; 58:1142–1152
    [Google Scholar]
  11. Harper W.M. Statistics 1965 London: Macdonalds & Evans;
    [Google Scholar]
  12. Hayashi K. A rapid determination of sodium dodecyl sulfate with methylene blue. Anal Biochem 1975; 67:503–506
    [Google Scholar]
  13. Hsu Y.-C. Detergent (SLS)-splitting enzyme from Pseudomonas. Nature 1963; 200:1091–1092
    [Google Scholar]
  14. Humphries M., Jaworzyn J.F., Cantwell J.B. The effect of a range of biological polymers and synthetic surfactants on the adhesion of a marine Pseudomonas sp. strain NCMB 2021 to hydrophilic and hydrophobic surfaces. FEMS Microbiol Ecol 1986; 38:299–308
    [Google Scholar]
  15. Humphries M., Jaworzyn J.F., Cantwell J.B., Eakin A. The use of non-ionic ethoxylated and propoxylated surfactants to prevent the adhesion of bacteria to solid surfaces. FEMS Microbiol Lett 1987; 42:91–101
    [Google Scholar]
  16. Klotz S.A., Drutz D.S., Zajic J.E. Factors governing adherence of Candida species to plastic surfaces. Infect Immun 1985; 50:97–101
    [Google Scholar]
  17. Lawrence J.R., Delaquis P.J., Korber D.R., Caldwell D.E. Behavior of Pseudomonas fluorescens within the hydrodynamic boundary layers of surface microenvironments. Microb Ecol 1987; 14:1–14
    [Google Scholar]
  18. van Loosdrecht M.C.M., Lyklema J., Norde W., Schraa G., Zehnder A.J.B. The role of bacterial cell wall hydro-phobicity in adhesion. Appl Environ Microbiol 1987; 53:1893–1897
    [Google Scholar]
  19. Marchesi J.R., House W.A., White G.F., Russell N.J., Farr I.S. A comparative study of the adsorption of linear alkyl sulphates and alkylbenzene sulphonates on river sediments. Colloids Surf 1991a; S3:63–78
    [Google Scholar]
  20. Marchesi J.R., Russell N.J., White G.F., House W.A. Effects of surfactant adsorption and biodegradability on the distribution of bacteria between sediments and water in a freshwater microcosm. Appl Environ Microbiol 1991b; 57:2507–2513
    [Google Scholar]
  21. Marshall K.C., Cruickshank R.H. Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Arch Mikrobiol 1973; 91:29–40
    [Google Scholar]
  22. McCourtie J., Mac Farlane T.W., Samaranayake L.P. Effect of chlorhexidine gluconate on the adherence of Candida species to denture acrylic. J Med Microbiol 1985; 20:97–104
    [Google Scholar]
  23. McEldowney S., Fletcher M. Variability of the influence of physicochemical factors affecting bacterial adhesion to polystyrene substrate. Appl Environ Microbiol 1986; 52:460–465
    [Google Scholar]
  24. Oberbremer A., Müller-Hurtig R., Wagner F. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl Microbiol Biotechnol 1990; 32:485–489
    [Google Scholar]
  25. Paul J.H., Jeffrey W.H. The effect of surfactants on the attachment of estuarine and marine bacteria to surfaces. Can J Microbiol 1985; 31:224–228
    [Google Scholar]
  26. Payne W.J., Feisal V.E. Bacterial utilization of dodecyl sulphate and dodecylbenzene sulfonate. Appl Microbiol 1963; 11:339–344
    [Google Scholar]
  27. Payne W.J., Painter B.D. Resolution by acrylamide gel electrophoresis of alkyl sulphatases and alcohol dehydrogenases. Microbios 1971; 3:199–206
    [Google Scholar]
  28. Rosenberg E., Gottlieb A., Rosenberg M. Inhibition of bacterial adherence to hydrocarbons and epithelial cells by emulsan. Infect Immun 1983; 39:1024–1028
    [Google Scholar]
  29. Thomas O.R.T. Development of solid support systems for the degradation of surfactants 1987 PhD Thesis, University of Wales;
    [Google Scholar]
  30. Thomas O.R.T., White G.F. Metabolic pathway for the biodegradation of sodium dodecyl sulfate by Pseudomonas sp. C12B. Biotech Appl Biochem 1989; 11:318–327
    [Google Scholar]
  31. Vanhaecke J., Remon J., Moors M., Raes F., de Rudder D., van Peteghem A. Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity. Appl Environ Microbiol 1990; 56:788–795
    [Google Scholar]
  32. White G.F., Russell N.J. Biodegradation of anionic surfactants and related molecules. In Biochemistry of Microbial Degradation 1993 Edited by Ratledge C. London: Kluwer; pp 143–177
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-11-2999
Loading
/content/journal/micro/10.1099/13500872-140-11-2999
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error