1887

Abstract

Summary: Enterotoxin production in is both strain dependent and sporulation associated. Underlying these phenotypic observations must lie a genetic and molecular explanation and the principal keys will be held within the DNA sequence both upstream and downstream of the structural gene cpe. In accordance with the above we have sequenced 4·1 kbp of DNA upstream of in the type strain NCTC 8239. A region of DNA extending up to 1·5 kb 5′ to is conserved in all enterotoxin-positive strains. This region contains a putative ORF with substantial homology to an ORF in the IS200 insertion element and, in addition, contains multiple perfect consensus DNA-binding sequences for the transition state regulator Hpr. The detailed structural elements revealed by the sequence analysis are presented and used to develop a new perspective on the molecular basis of enterotoxin production in this important food-poisoning bacterium.

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1994-01-01
2021-05-16
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References

  1. Alouf J.E., Knὅll H., K’hler W. The family of mitogenic, shock-inducing and superantigenic toxins from staphylococci and streptococci. In Sourcebook of Bacterial Protein Toxins 1991 Edited by Alouf J.E., Freer J.H. London: Academic Press; pp. 367–414
    [Google Scholar]
  2. Altschul S., Gish W., Miller W., Myers E., Lipman D. A basic logical alignment search tool.. J Mol Biol 1990; 215:403–410
    [Google Scholar]
  3. Ansorge W., Sproat B.S., Stegeman J., Schwager D. A non radioactive automated method for DNA sequence determination. J Biochem Biophys Methods 1986; 13:315–323
    [Google Scholar]
  4. Ansorge W., Sproat B.S., Stegeman J., Schwager D., Zenke M. Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Res 1987; 15:4593–4602
    [Google Scholar]
  5. Bowness P., Moss P.A., Tranter H., Bell J.I., Mc Michael A.J. Clostridium perfringens enterotoxin is a superantigen reactive with human T cell receptor Vb6.9 and Vb22.. j.Exp Med 1992; 176:893–896
    [Google Scholar]
  6. Canard B., Saint-Joanis B., Cole S.T. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol Microbiol 1992; 6:142–1429
    [Google Scholar]
  7. Duncan C.L., Strong D.H., Sebald M. Sporulation and enterotoxin production by mutants of Clostridium perfringens. J Bacteriol 1972; 110:378–391
    [Google Scholar]
  8. Gibert I., Carroll K., Hillyard D.R., Barbé J., Casadesus J. IS200 is not a member of the IS600 family of insertion sequences. Nucleic Acids Res 1991; 19:1343
    [Google Scholar]
  9. Granum P.E. Clostridium perfringens toxins involved in food poisoning. Int J Food Microbiol 1990; 10:101–112
    [Google Scholar]
  10. Granum P.E., Stewart G.S. A. B. 1993; Molecular biology of Clostridium perfringens enterotoxin. In Genetics and Molecular Biology of Anaerobic Bacteria Edited by Sebald M. New York: Springer; pp. 235–247
    [Google Scholar]
  11. Granum P.E., Telle W., Olsvik O., Stavn A. Enterotoxin formation by Clostridium perfringens during sporulation and vegetative growth. Int J Food Microbiol 1984; 1:43–49
    [Google Scholar]
  12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol 1983; 166:557–580
    [Google Scholar]
  13. Hanna P.C., Wnek A.P., Mc Clane B.A. Molecular cloning of the 3’ half of the Clostridium perfringens enterotoxin gene and demonstration that this region encodes receptor-binding activity. J Bacterial 1989; 171:6815–6820
    [Google Scholar]
  14. Iwanejko L.A., Routiedge M.N., Stewart G.S.A. B. loning in Escherichia coli of the enterotoxin gene from Clostridium perfringens type A. J Gen Microbiol 1989; 135:903–909
    [Google Scholar]
  15. Kallio P.T., Fagelson J.E., Hoch J.A., Strauch M.A. he transition state regulator Hpr of Bacillus subtilis is a DNA- binding protein. J Biol Chem 1991; 266:13411–13417
    [Google Scholar]
  16. Kristensen T., Voss H., Schwager J., Stegemann J., Sproat B., Ansorge W. T7 DNA polymerase in automated dideoxy sequencing. Nucleic Acids Res 1988; 16:3487–3496
    [Google Scholar]
  17. Labbe R.G., Duncan C.L. Evidence for stable messenger ribonucleic acid during sporulation and enterotoxin synthesis by Clostridium perfringens type A. J Bacteriol 1977; 129:843–849
    [Google Scholar]
  18. Pearson W., Lipman D. Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 1988; 85:2444–2448
    [Google Scholar]
  19. Perego M., Hoch J.A. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis. J Bacteriol 1988; 170:2560–2567
    [Google Scholar]
  20. Pitcher D.D., Saunders N.A., Owen R.J. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8:151–156
    [Google Scholar]
  21. Rosenberg A.H., Simon M.N., Studier W.F., Roberts R.J. Survey and mapping of endonuclease cleavage sites in bacteriophage T7 DNA. J Mol Biol 1979; 135:907–915
    [Google Scholar]
  22. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Sebald M. 1993 Genetics and Molecular Biology of Anaerobic Bacteria. New York: Springer;
    [Google Scholar]
  24. Sherratt D. 1989; Tn3 and related transposable elements: site specific recombination and transposition. In Mobile DNA Edited by Berg D.E., Howe M.M. Washington, DC: American Society for Microbiology; pp. 163–184
    [Google Scholar]
  25. Skjelkvåle R., Duncan C.L. Enterotoxin formation by different toxigenic types of Clostridium perfringens. Infect Immun 1975; 11:563–575
    [Google Scholar]
  26. Skjelkvåle R., Stringer M.F., Smart J.L. Enterotoxin production by lecithinase-positive and lecithinase-negative Clostridium perfringens isolated from food poisoning outbreaks and other sources. J Appl Microbiol 1979; 47:329–339
    [Google Scholar]
  27. Strauch M.A., Hoch J.A. Transition-state regulators: sentinels of Bacillus subtilis post-exponential gene expression. Mol Microbiol 1993; 7:337–342
    [Google Scholar]
  28. Stewart G.S.A.B., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression. Plasmid 1986; 15:172–181
    [Google Scholar]
  29. Van Damme-Jongsten M., Wernars K., Notermans S. loning and sequencing of the Clostridium perfringens enterotoxin gene. Antonie Leeuwenhoek 1989; 56:181–190
    [Google Scholar]
  30. Yanisch-Perron C., Vieira J., Messing J. Improved Ml3 phage cloning vectors and host strains: nucleotide sequence of the M13mpl8 and pUC19 vectors. Gene 1985; 33:103–119
    [Google Scholar]
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