1887

Abstract

Summary: A number of non-replicative, integrational plasmids have been introduced into the chromosome of the NCIMB 8052 strain of . Integration occurred via homologous recombination, apparently by a Campbell-like mechanism. For plasmids containing internal fragments of or , insertion was mutagenic leading, respectively, to an inability to use sorbitol as sole source of carbon for energy and growth or an inability to form endospores. One insertion was used to determine the map location of a randomly cloned DNA fragment and another underwent amplification in the bacterial chromosome. These results suggest that integrational plasmids will be very useful tools for genetic analysis in . To our knowledge, this is the first demonstration of gene transfer involving homologous recombination with the chromosome of any species of .

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1994-01-01
2022-01-20
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References

  1. Azeddoug H., Hubert J., Reysset G. 1992; Stable inheritance of shuttle vectors based on plasmid pIM13 in a mutant strain of Clostridium acetobutylicum. J Gen Microbiol 138:1371–1378
    [Google Scholar]
  2. Bertram J., Kuhn A., DUrre P. 1990; Tn916-induced mutants of Clostridium acetobutylicum defective in regulation of solvent formation. Arch Microbiol 153:373–377
    [Google Scholar]
  3. Biswas I., Vagner V., Ehrlich S.D. 1992; Efficiency of homologous intermolecular recombination at different locations on the JBacillus subtilis chromosome. J Bacteriol 174:5593–5596
    [Google Scholar]
  4. Biswas I., Gruss A., Ehrlich S.D., Maguin E. 1993; High- efficiency gene inactivation and replacement system for Gram-positive bacteria. J Bacteriol 175:3628–3635
    [Google Scholar]
  5. Azeddoug H., Hubert J., Reysset G. 1992; Stable inheritance of shuttle vectors based on plasmid pIM13 in a mutant strain of Clostridium acetobutylicum. J Gen Microbiol 138:1371–1378
    [Google Scholar]
  6. Biswas I., Vagner V., Ehrlich S.D. 1992; Efficiency of homologous intermolecular recombination at different locations on the J Bacillus subtilis chromosome. J Bacteriol 174:5593–5596
    [Google Scholar]
  7. Biswas I., Gruss A., Ehrlich S.D., Maguin E. 1993; High-efficiency gene inactivation and replacement system for Gram-positive bacteria. J BacteriolSargent 175:3628–3635
    [Google Scholar]
  8. Boyer H.W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
    [Google Scholar]
  9. Campbell A.M. 1962 Episomes. Adv Genet 11:101–146
    [Google Scholar]
  10. Chassy B.M., Murphy C.M. 1993; Eactococcus and Eactobacillus. In Bacillus subtilis and other Gram-positive Bacteria: Biochemistry, Physiology and Molecular Genetics pp 65–82 Edited by Sonenshein A. L., Hoch J.A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Cox M.M., Lehman I.R. 1987; Enzymes of general recombination. Annu Rev Biochem 56:229–262
    [Google Scholar]
  12. Del Sal G., Manfioletti G., Schneider C. 1988; A one tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res 16:9878
    [Google Scholar]
  13. Dowds B.C.A., O'Kane C., Gormley E., McConnell D.J., Devine K.M. 1988; Integrating plasmids in the genetic engineering of bacilli. In Recombinant DNA and Bacterial Fermentation pp 137–156 Edited by Thomson J. A. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  14. Gruss A., Moretto V., Ehrlich S. D., Duwat P., Dabert P. 1991; GC-rich DNA sequences block homologous recombination in vitro. J Biol Chem 266:6667–6669
    [Google Scholar]
  15. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–579
    [Google Scholar]
  16. Hedges R.W., Jacob A.E. 1974; Transposition of ampicillin resistance from RP4 to other replicons. Mol & Gen Genet 132:31–34
    [Google Scholar]
  17. Jannifere L., Niaudet B., Pierre E., Ehrlich S.D. 1985; Stable gene amplification in the chromosome of Bacillus subtilis. Gene 40:47–55
    [Google Scholar]
  18. Jones D.T., Woods D.R. 1986; Acetone-butanol fermentation revisited. Microbiol Rev 50:484–524
    [Google Scholar]
  19. Khasanov F. K., Zvingila D. J., Zainullin A.A., Prozorov A.A. 1992; Homologous recombination between plasmid and chromosomal DNA in Bacillus subtilis requires approximately 70 bp of homology. Mol & Gen Genet 234:494–497
    [Google Scholar]
  20. Kieser T., Hopwood D.A. 1991; Genetic manipulation of Streptomyces: integrating vectors and gene replacement. Methods Enzymol 204:430–458
    [Google Scholar]
  21. Leenhouts K. J., Kok J., Venema G. 1989; Campbell-like integration of heterologous plasmid DNA into the chromosome of Eactococcus lactis subsp. lactis. Appl Environ Microbiol 55:394–400
    [Google Scholar]
  22. Maguin E., Duwat P., Hege T., Ehrlich S. D., Gruss A. 1992; New thermosensitive plasmid for Gram-positive bacteria. J Bacteriol 174:5633–5638
    [Google Scholar]
  23. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. J Mol Biol 53:159–162
    [Google Scholar]
  24. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: A Eahoratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Mermelstein L. D., Welker N. E., Bennett G.N., Papoutsakis E.T. 1992; Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Biotechnol 10:190–195
    [Google Scholar]
  26. Michel B., Niaudet B., Ehrlich S.D. 1983; Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and dimeric plasmids. Plasmid 10:1–10
    [Google Scholar]
  27. Michel B., Ehrlich S.D. 1984; Recombination efficiency is a quadratic function of the length of homology during plasmid transformation of Bacillus subtilis protoplasts and Escherichia coli competent cells. EMBO J 3:2879–2884
    [Google Scholar]
  28. Minton N. P., Swinfield T.-J., Brehm J. K., Whelan S.M., Oultram J.D. 1993; Vectors for use in Clostridium acetobutylicum. In Genetics and Molecular Biology of Anaerobic Bacteria pp 120–140 Edited by Sebald M. New York: Springer;
    [Google Scholar]
  29. Noirot P.H., Petit M.-A., Ehrlich S.D. 1987; Plasmid replication stimulates DNA recombination in Bacillus subtilis. J Mol Biol 196:39–48
    [Google Scholar]
  30. Norrander J., Kempe T., Messing J. 1983; Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 27:101–106
    [Google Scholar]
  31. O'Brien R.W., Morris J.G. 1971; Oxygen and the growth and metabolism of Clostridium acetobutylicum. J Gen Microbiol 68:307–318
    [Google Scholar]
  32. Oultram J. D., Loughlin M., Swinfield T.-J., Brehm J. K., Thompson D. E., Minton N.P. 1988; Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation. FEMS Microbiol Eett 56:83–88
    [Google Scholar]
  33. Perego M. 1993; Integrational vectors for genetic manipulation in Bacillus subtilis. In Bacillus subtilis and other Gram-positive Bacteria: Biochemistry, Physiology and Molecular Genetics pp 615–624 Edited by Sonenshein A.L., Hoch J.A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Piggot P.J., Curtis C.A. M. 1987; Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions. J Bacteriol 169:1260–1266
    [Google Scholar]
  35. Piggot P. J., Curtis C. A. M., de Lencastre H. 1984; Use of integrational plasmid vectors to demonstrate the polycistronic nature of a transcriptional unit (spoil A) required for sporulation of Bacillus subtilis. J Gen Microbiol 130:2123–2136
    [Google Scholar]
  36. Sargent M.G., Bennett M.F. 1985; Amplification of a major membrane-bound DNA sequence of Bacillus subtilis. J Bacteriol 161:589–595
    [Google Scholar]
  37. Southern E. 1975; Detection of specific sequences among DN A fragments separated by gel electrophoresis. J Mol Biol 98:503 –517
    [Google Scholar]
  38. Stojanovic S., Hranueli D., Young M. 1992; Evidence that recombination between reiterated sequences in the Bacillus subtilis chromosome does not occur via unequal crossing over. Biochimie 74:713–721
    [Google Scholar]
  39. Twigg A.J., Sherratt D. 1980; Trans-complementable mutants of plasmid Col El. Nature 283:216–218
    [Google Scholar]
  40. Vagner V., Ehrlich S.D. 1988; The efficiency of homologous recombination varies along the Bacillus subtilis chromosome. J Bacteriol 170:3978–3982
    [Google Scholar]
  41. Wahl G. M., Stern M., Stark G.R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687
    [Google Scholar]
  42. Wilkinson S.R., Young M. 1993; Wide diversity of genome size among different strains of Clostridium acetobutylicum. J Gen Microbiol 139:1069–1076
    [Google Scholar]
  43. Williams D. R., Young D. I., Young M. 1990; Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum. J Gen Microbiol 136:819–826
    [Google Scholar]
  44. Woolley R. C., Pennock A., Ashton R. J., Davies A., Young M. 1989; Transfer of Tn 1545 and Tn916 to Clostridium acetobutylicum. Plasmid 22:169–174
    [Google Scholar]
  45. Young M. 1984; Gene amplification in Bacillus subtilis. J Gen Microbiol 130:1613–1621
    [Google Scholar]
  46. Young M., Cole S.T. 1993; Clostridium. In Bacillus subtilis and other Gram-positive Bacteria: Biochemistry, Physiology and Molecular Genetics pp 35–52 Edited by Sonenshein A.L., Hoch J.A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  47. Young M., Ehrlich S.D. 1989; Stability of reiterated sequences in the Bacillus subtilis chromosome. J Bacteriol 171:2653–2656
    [Google Scholar]
  48. Young M., Hranueli D. 1988; Chromosomal gene amplification in Gram-positive bacteria. In Recombinant DNA and Bacterial Fermentation pp 157–200 Edited by Thomson J. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  49. Young M., Staudenbauer W.L., Minton N.P. 1989; Recent advances in the genetics of the clostridia. FEAIS Microbiol Rev 63:301–325
    [Google Scholar]
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