RT Journal Article SR Electronic(1) A1 Kokjohn, Tyler A. A1 Miller, Robert V.YR 1994 T1 IncN plasmids mediate UV resistance and errorprone repair in Pseudomonas aeruginosa PAO JF Microbiology, VO 140 IS 1 SP 43 OP 48 DO https://doi.org/10.1099/13500872-140-1-43 PB Microbiology Society, SN 1465-2080, AB Summary: While it seems likely that the ability to induce the expression of recA-controlled genes is nearly universal among the eubacteria, the expression of plasmid-borne ultraviolet (UV)-resistance and mutagenesis genes seems to be species-dependent in a complex fashion. Some plasmids encoding UV-resistance and mutagenesis functions only express these phenotypes in a select number of bacterial species. Several UV-resistance plasmids that express these functions in Escherichia coli are either unstable or simply do not express the UV-resistance-mutagenesis phenotype in Pseudomonas aeruginosa. In order to clarify the role of these plasmids in microbial ecology, we have undertaken a study of the ability of the well-characterized UV-resistance IncN plasmids pKM101 and R46 to express the UV-resistance phenotype in P. aeruginosa. In addition, we have examined the IncP plasmids RP4 and R68.45, observed to confer a UV-resistant phenotype upon Myxococcus xanthus, for the ability to express this phenotype in P. aeruginosa. Our experiments reveal that while pKM101 and R46 transfer to P. aeruginosa at a very low frequency, these plasmids, once transferred, are maintained and clearly support the expression of the UV-resistance and mutagenesis phenotype observed in E. coli. Studies of plasmids R68.45 and RP4 in P. aeruginosa revealed that they do not express UV-resistance functions in this species. UV-resistance plasmids may play an important role in the natural ecology of bacterial habitats exposed to solar radiation or to various DNA-damaging natural and man-made chemicals., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-1-43