@article{mbs:/content/journal/micro/10.1099/13500872-140-1-43, author = "Kokjohn, Tyler A. and Miller, Robert V.", title = "IncN plasmids mediate UV resistance and errorprone repair in Pseudomonas aeruginosa PAO", journal= "Microbiology", year = "1994", volume = "140", number = "1", pages = "43-48", doi = "https://doi.org/10.1099/13500872-140-1-43", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-1-43", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Pseudomonas aeruginosa", keywords = "Weigle reactivation/mutagenesis. recA", keywords = "UV-resistance plasmids", keywords = "SOS response", abstract = "Summary: While it seems likely that the ability to induce the expression of recA-controlled genes is nearly universal among the eubacteria, the expression of plasmid-borne ultraviolet (UV)-resistance and mutagenesis genes seems to be species-dependent in a complex fashion. Some plasmids encoding UV-resistance and mutagenesis functions only express these phenotypes in a select number of bacterial species. Several UV-resistance plasmids that express these functions in Escherichia coli are either unstable or simply do not express the UV-resistance-mutagenesis phenotype in Pseudomonas aeruginosa. In order to clarify the role of these plasmids in microbial ecology, we have undertaken a study of the ability of the well-characterized UV-resistance IncN plasmids pKM101 and R46 to express the UV-resistance phenotype in P. aeruginosa. In addition, we have examined the IncP plasmids RP4 and R68.45, observed to confer a UV-resistant phenotype upon Myxococcus xanthus, for the ability to express this phenotype in P. aeruginosa. Our experiments reveal that while pKM101 and R46 transfer to P. aeruginosa at a very low frequency, these plasmids, once transferred, are maintained and clearly support the expression of the UV-resistance and mutagenesis phenotype observed in E. coli. Studies of plasmids R68.45 and RP4 in P. aeruginosa revealed that they do not express UV-resistance functions in this species. UV-resistance plasmids may play an important role in the natural ecology of bacterial habitats exposed to solar radiation or to various DNA-damaging natural and man-made chemicals.", }