1887

Abstract

Summary: Slow-growing mycobacteria have a single ribosomal RNA () operon, with the genes for 16S, 23S and 5S rRNA being present in that order. The transcription start site of the operon of was identified in PCR methodology was used to amplify parts of the operon, namely the leader region and the spacer-1 region separating the 16S rRNA and 23S rRNA genes of and . The amplified DNA was sequenced. The sequence data, together with those obtained previously for and , were used to identify putative antitermination signals and RNase III processing sites within the leader region. Notable features include a highly conserved Box B element and a sequence of 31 nucleotides which is common to all eight slow-growers which were scrutinized. A secondary structure for mycobacterial precursor-16S rRNA was devised, based on sequence homologies and homologous nucleotide substitutions. The 18 nucleotides at the 5’-end of spacer-1 have the capacity of binding sequences close to the 5-and 3’-ends of mature 16S rRNA, suggesting that secondary structure is important to the maturation process. All the slow-growers, including , conform to the same scheme of secondary structure. The scheme proposed for is a variant of the main theme. The leader and spacer sequences may prove a useful supplement to 16S rRNA sequences in establishing phylogenetic relationships between very closely related species. appears to be a close relative of .

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1994-01-01
2021-10-22
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References

  1. Apirion D., Miczak A. 1993; RNA processing in prokaryotic cells. Bio Essays 15:113–120
    [Google Scholar]
  2. Berg K. L., Squires C., Squires C.L. 1989; Ribosomal RNA operon anti-termination: function of leader and spacer region Box B-Box A sequences and their conservation in diverse microorganisms. J Mol Biol 209:345–358
    [Google Scholar]
  3. Bercovier H., Kafri O., Sela S. 1986; Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys lies Commun 136:1136–1141
    [Google Scholar]
  4. Bremer H., Dennis P.P. 1987; Modulation of chemical composition and other parameters of the cell growth rate. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp 1527–1542 Edited by Neidhardt F. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Bruckner D.A., Colonna P. 1993; Nomenclature for aerobic and faculative bacteria. Clin Infect Dis 16:598–605
    [Google Scholar]
  6. Clark J.M. 1988; Novel non-templated nucleotide addition reactions catalyzed by prokaryotic and eukaryotic DNA polymerases. Nucleic Acids Res 16:9677–9686
    [Google Scholar]
  7. Cox R. A., Kempsell K., Fairdough L., Colston M.J. 1991; The 16S ribosomal RNA of Mycobacterium leprae contains a unique sequence which can be used for identification by the polymerase chain reaction. J Med Microbiol 35:284–290
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  9. Dubos R.i., Davis B.D. 1946; Factors affecting the growth of tubercle bacilli in liquid media. J Exp Med 83:409–423
    [Google Scholar]
  10. Estrada-G I.C. E., Colston M.J., Cox R.A. 1989; Determination and evolutionary significance of nucleotide sequences near to the 3’-end of 16S ribosomal RNA of mycobacteria. FEMS Microbiol Eett 61:285–290
    [Google Scholar]
  11. Iwami M., Muto A., Yamao F., Osawa S. 1984; Nucleotide sequence of the rrn B 16S ribosomal RNA gene from Mycoplasma capricolum . Mol & Gen Genet 196:317–322
    [Google Scholar]
  12. Kempsell K. E., Ji Y-E., Estrada-G I.C. E., Colston M.J., Cox R.A. 1992; The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA. J Gen Microbiol 138:1717–1727
    [Google Scholar]
  13. Li S. C., Squires C.L., Squires C. 1984; Antitermination of E. coli rRNA transcription is caused by a control region segment containing nut-Wkc sequences. Cell 38:851–860
    [Google Scholar]
  14. Liesack W., Pitulle C., Sela S., Stackebrandt E. 1990; Nucleotide sequence of the 16S rRNA from Mycobacterium leprae . Nucleic Acids Res 18:5558
    [Google Scholar]
  15. Liesack W., Sela S., Bercovier H., Pitulle C., Stackebrandt E. 1991; Complete nucleotide sequence of the Mycobacterium leprae 23S and 5S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes. FEBS Lett 281:114–118
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Meissner G., Schroder K.-H. 1975; Relationship between Mycobacterium simiae and Mycobacterium habana . Am Rev Respir Dis 111:196–200
    [Google Scholar]
  18. Ogasawara N., Moriya S., Yoshikawa H. 1983; Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. Nucleic Acids Res 11:6301–6318
    [Google Scholar]
  19. Pernodet J.-L., Boocard F., Alegre M.-T., Gagnat J., Guerineau M. 1989; Organization and nucleotide sequence analysis of a ribosomal RNA cluster from Streptomyces ambofaciens. . Gene 59:33–46
    [Google Scholar]
  20. Portaels F. 1980; Unclassified mycobacterial strains susceptible to dapsone isolated from the environment in central Africa. Int I Lepr 48:330
    [Google Scholar]
  21. Rogall T., Flohr T., Böttger E.C. 1990a; Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol 136:1915–1920
    [Google Scholar]
  22. Rogall T., Wolters J., Flohr T., Bottger E.C. 1990b; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . Int J Syst Bacterial 40:323–330
    [Google Scholar]
  23. Saiki R. K., Gelfand D. H., Stoffel S., Sharf S. J., Higuchi R., Horn G.T., Mullis K.B., Erlich H.A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  24. Sela S., Clark-Curtiss J.E. 1991; Cloning and characterization of the Mycobacterium leprae putative ribosomal RNA promoter in Escherichia coli . Gene 98:123–127
    [Google Scholar]
  25. Sela S., Clark-Curtiss J.E., Bercovier H. 1989; Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae . J Bacteriol 111:70–73
    [Google Scholar]
  26. Shine J., Dalgarno L. 1974; The 3’-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346
    [Google Scholar]
  27. Singh N.B., Lowe A.C. R. E., Rees R. J. W., Colston M.J. 1989; Vaccination of mice against Mycobacterium leprae infection. Infect Immun 57:653–655
    [Google Scholar]
  28. Suzuki Y., Nagata A., Ono Y., Yamada I. 1988; Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J Bacteriol 170:1631–1636
    [Google Scholar]
  29. Taschke C., Herrmann R. 1986; Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae . Mol & Gen Genet 205:434–441
    [Google Scholar]
  30. Teske A., Wolters J., Bbttger E.C. 1991; The 16S rRNA nucleotide sequence of Mycobacterium leprae: phylogenetic position and development of DNA probes. FEMS Microbiol Lett 80:231–238
    [Google Scholar]
  31. ValdiviaAlvarez J., Suarez Mendez S., Echemendia Font M. 1971; Mycobacterium habana: probable especie dentro de las micobacterias no clasificadas. Bol Hig Epidemiol 9:65–73
    [Google Scholar]
  32. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76:615–619
    [Google Scholar]
  33. Wezel G.P.V., Vijgenboom E., Bosch L. 1991; A comparative study of the ribosomal RNA operons of Streptomyces coelicolor A3(2) and sequence analysis of rrn A. . Nucleic Acids Res 19:4399–4403
    [Google Scholar]
  34. Winder F.G., Rooney S.A. 1970; Effects of nitrogenous components of the medium on the carbohydrate and nucleic acid content of Mycobacterium tuberculosis BCG. J Gen Microbiol 63:29–39
    [Google Scholar]
  35. Young R.A., Steitz J.A. 1978; Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA. Proc Natl Acad Sci USA 75:3503–3597
    [Google Scholar]
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