1887

Abstract

Summary: Slow-growing mycobacteria have a single ribosomal RNA () operon, with the genes for 16S, 23S and 5S rRNA being present in that order. The transcription start site of the operon of was identified in PCR methodology was used to amplify parts of the operon, namely the leader region and the spacer-1 region separating the 16S rRNA and 23S rRNA genes of and . The amplified DNA was sequenced. The sequence data, together with those obtained previously for and , were used to identify putative antitermination signals and RNase III processing sites within the leader region. Notable features include a highly conserved Box B element and a sequence of 31 nucleotides which is common to all eight slow-growers which were scrutinized. A secondary structure for mycobacterial precursor-16S rRNA was devised, based on sequence homologies and homologous nucleotide substitutions. The 18 nucleotides at the 5′-end of spacer-1 have the capacity of binding sequences close to the 5-and 3′-ends of mature 16S rRNA, suggesting that secondary structure is important to the maturation process. All the slow-growers, including , conform to the same scheme of secondary structure. The scheme proposed for is a variant of the main theme. The leader and spacer sequences may prove a useful supplement to 16S rRNA sequences in establishing phylogenetic relationships between very closely related species. appears to be a close relative of .

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/content/journal/micro/10.1099/13500872-140-1-123
1994-01-01
2019-11-20
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-1-123
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