SUMMARY: The thiosulphate-oxidizing system of was destroyed by treatment with proteolytic enzymes, phospholipase A, sodium deoxycholate and lysolecithin, indicating essential roles for phospholipids and proteins in the membrane complex. The complex was partially resolved with trypsin and sodium deoxycholate into component enzymes involved in the oxidation of thiosulphate to sulphate: thiosulphate-cleaving enzyme (rhodanese; EC, sulphur-oxidizing enzyme (EC, sulphite:cytochrome oxidoreductase (EC and cytochrome oxidase (EC All attempts to reconstitute the thiosulphate-oxidizing system from these component enzymes were unsuccessful. A possible mechanism for thiosulphate oxidation by the complex is discussed.


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