Host Modification and Restriction with a Mycobacteriophage Isolated from a Pseudolysogenic Free

Abstract

Summary: A pseudolysogenic and its phage 630 are described. Phage 630 is the first mycobacteriophage reported to be resistant to the non-polar solvents chloroform, dioxan and diethyl ether. The phage had a latent period of 75 min, a rise period of 90 min and a burst size of 51. Evidence is presented for host modification and restriction. Phage 630A, grown on host strain -630 Rg, plated on the alternative host 607 with an efficiency of plating (e.o.p.) of 10. Phage 630B, grown on host , plated with an e.o.p. of 10 on the alternative host -630 Rg. Phages 630A and 630B adsorbed equally well on their alternative hosts and on their indicator host strains. The progeny of plaques from initial platings on the alternative host, when grown in the alternative host, exhibited a marked reduction in e.o.p. on their original host.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-99-2-389
1977-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/99/2/mic-99-2-389.html?itemId=/content/journal/micro/10.1099/00221287-99-2-389&mimeType=html&fmt=ahah

References

  1. Adams M.H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Arber W. 1965; Host controlled modification of bacteriophage. Annual Review of Microbiology 19:365–378
    [Google Scholar]
  3. Arber W., Dussoix D. 1962; Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage λ. Journal of Molecular Biology 5:18–36
    [Google Scholar]
  4. Baess I. 1971; Report on a pseudolysogenic mycobacterium and a review of the literature concerning pseudolysogeny. Acta pathologica et microbiologica scandinavica B79:428–434
    [Google Scholar]
  5. Bowman B.U. Jr 1958; Quantitative studies on some mycobacterial phage host systems. Journal of Bacteriology 76:52–62
    [Google Scholar]
  6. Bowman B.U. Jr 1969; Effect of chloroform on mycobacteriophages. Microbios 4:347–352
    [Google Scholar]
  7. Bowman B.U., Newman H.A.I., Moritz J.M., Koehler R.M. 1973; Properties of mycobacterio-phage DS6A. II. Lipid composition. American Review of Respiratory Diseases 107:42–49
    [Google Scholar]
  8. Grange J.M., Bird R.G. 1975; The nature and incidence of lysogeny in Mycobacterium fortuitum. Journal of Medical Microbiology 8:215–223
    [Google Scholar]
  9. Hayes W. 1968 The Genetics of Bacteria and Their Viruses New York: John Wiley;
    [Google Scholar]
  10. Hubácek J., Kupkova H., Mohelská H. 1970; A special case of lysogeny in Mycobacterium avium. Folia microbiologica 15:341–346
    [Google Scholar]
  11. Jones W.D. Jr 1973; Studies on the bacteriophage of a naturally lysogenic Mycobacterium fortuitum. American Review of Respiratory Diseases 108:1438–1441
    [Google Scholar]
  12. Jones W.D. Jr David H.L. 1971; Inhibition by rifampin of mycobacteriophage D29 replication in its drug resistant host, Mycobacterium smegmatis atcc607. American Review of Respiratory Diseases 103:618–624
    [Google Scholar]
  13. Jones W.D. Jr Greenberg J. 1976; Use of phage F-øWJ-1 of Mycobacterium fortuitum to discern more phage types of Mycobacterium tuberculosis. Journal of Clinical Microbiology 3:324–326
    [Google Scholar]
  14. Jones W.D. Jr Kubica G.P. 1965; Differential colonial characteristics of mycobacteria on oleic acid-albumin and modified com meal agars. 2. Investigation of rapidly-growing mycobacteria. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) 196:53–67
    [Google Scholar]
  15. Jones W.D. Jr White A. 1968; Lysogeny in mycobacteria. I. Conversion of colony morphology, nitrate reductase activity, and Tween 80 hydrolysis of Mycobacterium sp. atcc607 associated with lysogeny. Canadian Journal of Microbiology 14:551–555
    [Google Scholar]
  16. Jones W.D. Jr David H.L., Beam R.E. 1970; The occurrence of lipids in mycobacteriophage D29 propagated in Mycobacterium smegatis atcc607. American Review of Respiratory Diseases 102:814–817
    [Google Scholar]
  17. Lwoff A. 1953; Lysogeny. Bacteriological Reviews 17:269–337
    [Google Scholar]
  18. Marion R.E., Bradley S.G. 1964; Derivation of new mycobacteriophage typing reagents by propagation on alternate hosts. American Review of Respiratory Diseases 89:674–676
    [Google Scholar]
  19. Nördström G., Grange J.M. 1974; Bacteriophage typing of Mycobacterium ranae (fortuiturn). The use of unadapted and adapted phages in the development of a typing system. Acta pathologica et microbiologica scandinavica B82:87–93
    [Google Scholar]
  20. Rado T.A., Bates J.H., Engel H.W.B., Mankiewicz E., Murohashi T., Mizuguchi Y., Sula L. 1975; World Health Organization studies on bacteriophage typing of mycobacteria. Subdivision of the species Mycobacterium tuberculosis. American Review of Respiratory Diseases 111:459–468
    [Google Scholar]
  21. Rado T.A., Bates J.H., Fitzhugh J.K. 1976; Host controlled restriction and modification of bacteriophage in Mycobacterium tuberculosis. Journal of General Virology 30:91–97
    [Google Scholar]
  22. Redmond W.B. 1963; Bacteriophages of the mycobacteria-a review. Advances in Tuberculosis Research 12:191–229
    [Google Scholar]
  23. Sellers M.I., Tokunaga T. 1970; Inactivation of mycobacteriophages by lipid solvents. In Host-Virus Relationships in Mycobacteria, Nocardia and Actinomyces pp. 134–143 Springfield, Illinois: Charles C. Thomas;
    [Google Scholar]
  24. White A., Knight V. 1954; Effect of TweenR 80 and serum on the interaction of mycobacteriophage D29 with certain mycobacterial species. American Review of Respiratory Diseases 77:134–145
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-2-389
Loading
/content/journal/micro/10.1099/00221287-99-2-389
Loading

Data & Media loading...

Most cited Most Cited RSS feed