Summary: A pseudolysogenic Mycobacterium chelonei and its phage ϕ630 are described. Phage ϕ630 is the first mycobacteriophage reported to be resistant to the non-polar solvents chloroform, dioxan and diethyl ether. The phage had a latent period of 75 min, a rise period of 90 min and a burst size of 51. Evidence is presented for host modification and restriction. Phage ϕ630A, grown on host strain M. cheloneif-630 Rg, plated on the alternative host M. smegmatisatcc607 with an efficiency of plating (e.o.p.) of 10−5. Phage ϕ630B, grown on host M. smegmatis, plated with an e.o.p. of 10−5 on the alternative host f-630 Rg. Phages ϕ630A and ϕ630B adsorbed equally well on their alternative hosts and on their indicator host strains. The progeny of plaques from initial platings on the alternative host, when grown in the alternative host, exhibited a marked reduction in e.o.p. on their original host.
ArberW.,
DussoixD.1962; Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage λ. Journal of Molecular Biology 5:18–36
BaessI.1971; Report on a pseudolysogenic mycobacterium and a review of the literature concerning pseudolysogeny. Acta pathologica et microbiologica scandinavica B79:428–434
BowmanB.U.,
NewmanH.A.I.,
MoritzJ.M.,
KoehlerR.M.1973; Properties of mycobacterio-phage DS6A. II. Lipid composition. American Review of Respiratory Diseases 107:42–49
JonesW.D.JrDavidH.L.1971; Inhibition by rifampin of mycobacteriophage D29 replication in its drug resistant host, Mycobacterium smegmatisatcc607. American Review of Respiratory Diseases 103:618–624
JonesW.D.JrGreenbergJ.1976; Use of phage F-øWJ-1 of Mycobacterium fortuitum to discern more phage types of Mycobacterium tuberculosis. Journal of Clinical Microbiology 3:324–326
JonesW.D.JrKubicaG.P.1965; Differential colonial characteristics of mycobacteria on oleic acid-albumin and modified com meal agars. 2. Investigation of rapidly-growing mycobacteria. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) 196:53–67
JonesW.D.JrWhiteA.1968; Lysogeny in mycobacteria. I. Conversion of colony morphology, nitrate reductase activity, and Tween 80 hydrolysis of Mycobacterium sp. atcc607 associated with lysogeny. Canadian Journal of Microbiology 14:551–555
JonesW.D.JrDavidH.L.,
BeamR.E.1970; The occurrence of lipids in mycobacteriophage D29 propagated in Mycobacterium smegatisatcc607. American Review of Respiratory Diseases 102:814–817
MarionR.E.,
BradleyS.G.1964; Derivation of new mycobacteriophage typing reagents by propagation on alternate hosts. American Review of Respiratory Diseases 89:674–676
NördströmG.,
GrangeJ.M.1974; Bacteriophage typing of Mycobacterium ranae (fortuiturn). The use of unadapted and adapted phages in the development of a typing system. Acta pathologica et microbiologica scandinavica B82:87–93
RadoT.A.,
BatesJ.H.,
EngelH.W.B.,
MankiewiczE.,
MurohashiT.,
MizuguchiY.,
SulaL.1975; World Health Organization studies on bacteriophage typing of mycobacteria. Subdivision of the species Mycobacterium tuberculosis. American Review of Respiratory Diseases 111:459–468
RadoT.A.,
BatesJ.H.,
FitzhughJ.K.1976; Host controlled restriction and modification of bacteriophage in Mycobacterium tuberculosis. Journal of General Virology 30:91–97
SellersM.I.,
TokunagaT.1970; Inactivation of mycobacteriophages by lipid solvents. In Host-Virus Relationships in Mycobacteria, Nocardia and Actinomyces pp. 134–143 Springfield, Illinois: Charles C. Thomas;
WhiteA.,
KnightV.1954; Effect of TweenR 80 and serum on the interaction of mycobacteriophage D29 with certain mycobacterial species. American Review of Respiratory Diseases 77:134–145