Respiratory Properties of Synchronous Cultures of H16 Prepared by a Continuous-flow Size Selection Method Free

Abstract

Summary: Synchronous cultures of ( 16) were prepared by a size selection method which gave high synchrony indices (0·70 to 0·85). Unlike the smooth exponential increase which was observed in exponentially growing cultures, the respiration rate [g-atom O min (ml culture)] of synchronous cultures exhibited a periodic increase which was composed of two ‘steps’ with rises centred at approximately 0·4 and 0·9 of the cell-cycle. The respiration of exponentially growing and synchronous cultures was stimulated by the uncoupling agent carbonyl cyanide -chlorophenylhydrazone; the degree of stimulation varied during the cell-cycle and was inversely related to the respiration rate. The observed discontinuous patterns of respiration are discussed with reference to the reported respiratory properties of other micro-organisms in synchronous cultures.

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/content/journal/micro/10.1099/00221287-99-2-383
1977-04-01
2024-03-28
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