SUMMARY: Synchronous cultures of ( H16) were prepared by a size selection method which gave high synchrony indices (0.70 to 0.85). Unlike the smooth exponential increase which was observed in exponentially growing cultures, the respiration rate [μg-atom O min (ml culture)] of synchronous cultures exhibited a periodic increase which was composed of two ‘steps’ with rises centred at approximately 0.4 and 0.9 of the cell-cycle. The respiration of exponentially growing and synchronous cultures was stimulated by the uncoupling agent carbonyl cyanide -chlorophenylhydrazone; the degree of stimulation varied during the cell-cycle and was inversely related to the respiration rate. The observed discontinuous patterns of respiration are discussed with reference to the reported respiratory properties of other micro-organisms in synchronous cultures.


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