1887

Abstract

Summary: Synchronous cultures of ( 16) were prepared by a size selection method which gave high synchrony indices (0·70 to 0·85). Unlike the smooth exponential increase which was observed in exponentially growing cultures, the respiration rate [g-atom O min (ml culture)] of synchronous cultures exhibited a periodic increase which was composed of two ‘steps’ with rises centred at approximately 0·4 and 0·9 of the cell-cycle. The respiration of exponentially growing and synchronous cultures was stimulated by the uncoupling agent carbonyl cyanide -chlorophenylhydrazone; the degree of stimulation varied during the cell-cycle and was inversely related to the respiration rate. The observed discontinuous patterns of respiration are discussed with reference to the reported respiratory properties of other micro-organisms in synchronous cultures.

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/content/journal/micro/10.1099/00221287-99-2-383
1977-04-01
2021-10-18
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References

  1. Beatrice M.C., Chappell J.B. 1974; Respiration-driven proton translocation in Hydrogenomonas eutropha H-16. Biochemical Society Transactions 2:151–153
    [Google Scholar]
  2. Blumenthal L.K., Zahler S.A. 1962; Index for measurement of synchronization of cell populations. Science 135:724
    [Google Scholar]
  3. Campbell A.C. 1957; Synchronization of cell division. Bacteriological Reviews 21:263–272
    [Google Scholar]
  4. Edwards C., Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata, by cell-size selection: changes in respiration and adenylate charge through the cell-cycle. Journal of General Microbiology 88:141–152
    [Google Scholar]
  5. Evans J.B. 1975; Preparation of synchronous cultures of Escherichia coli by continuous-flow size selection. Journal of General Microbiology 91:188–190
    [Google Scholar]
  6. Helmstetter C.E. 1969; Methods for studying the microbial division cycle. Methods in Microbiology 1:327–361
    [Google Scholar]
  7. James T.W. 1964 In Synchrony in Cell Division and Growth pp. 323–349 Zeuthen E. Edited by New York: Interscience.;
    [Google Scholar]
  8. Lloyd D., John L., Edwards C., Chagla A.H. 1975; Synchronous cultures of micro-organisms: large-scale preparation by continuous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  9. Maruyama Y., Yanagita T. 1956; Physical methods for obtaining synchronous cultures of Escherichia coli. Journal of Bacteriology 71:542–546
    [Google Scholar]
  10. Mitchison J.M. 1969; Enzyme synthesis in synchronous cultures. Science 165:657–663
    [Google Scholar]
  11. Mitchison J.M. 1971 The Biology of the Cell Cycle. London: Cambridge University Press.;
    [Google Scholar]
  12. Mitchison J.M., Vincent W.S. 1965; Preparation of synchronous cultures by sedimentation. Nature; London: 205987–989
    [Google Scholar]
  13. Poole R.K., Lloyd D. 1974; Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pombe 972h growing in the presence of glycerol. Biochemical Journal 164:141–148
    [Google Scholar]
  14. Poole R.K., Lloyd D., Kemp R.B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h. Journal of General Microbiology 77:209–220
    [Google Scholar]
  15. Probstt I., Schlegel H.G. 1976; Respiratory components and oxidase activities in Alcaligenes eutrophus. Biochimica et biophysica acta 440:412–428
    [Google Scholar]
  16. Schlegel H.G., Kaltwasser H., Gottschalk G. 1961; Submersverfahren zur Kultur wasserstoffoxy-dierender Bakterien: Wachstumsphysiologische Untersuchungen. Archiv für Mikrobiologie 38:209–222
    [Google Scholar]
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