1887

Abstract

Summary: Fluctuations in cell volume during exponential growth of k12 changed the effectiveness of the continuous-flow centrifugation method for preparing synchronous cultures. Rates of oxygen uptake in synchronous cultures were measured using an electrode system open to the atmosphere. In synchronous cultures of both the parental strain and an adenosine triphosphatase-deficient mutant, which was incapable of oxidative phosphorylation, respiration rates doubled during the cell cycle but oscillated with a periodicity of approximately half a cycle. Synchronous cultures of the parental strain growing on glycerol and Casamino acids showed a stepwise pattern of oxygen consumption. Continuous flow centrifugation did not markedly affect the increases in the numbers and respiration rates of cells in synchronous cultures. Respiratory oscillations also occurred on inoculation of a late-stationary phase culture into fresh medium, although synchronous division was not observed. The possible mechanisms underlying respiratory fluctuations under different growth conditions are discussed.

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/content/journal/micro/10.1099/00221287-99-2-369
1977-04-01
2021-10-24
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References

  1. Blumenthal L.K., Zahler S.A. 1962; Index for measurement of synchronization of cell populations. Science 135:724
    [Google Scholar]
  2. Chance B., Williams G.R. 1956; The respiratory chain and oxidative phosphorylation. Advances in Enzymology 17:65–134
    [Google Scholar]
  3. Chappell J.B. 1964; The oxidation of citrate, isocitrate and cis-aconitate by isolated mitochondria. Biochemical Journal 90:225–237
    [Google Scholar]
  4. Cutler R.G., Evans J.E. 1966; Synchronization of bacteria by a stationary-phase method. Journal of Bacteriology 91:469–476
    [Google Scholar]
  5. Degn H., Lilleør M., Iversen J.J.L. 1973; The occurrence of a stepwise-decreasing respiration rate during oxidative assimilation of different substrates by resting Klebsiella aerogenes in a system open to oxygen. Biochemical Journal 136:1097–1104
    [Google Scholar]
  6. Evans J.B. 1975; Preparation of synchronous cultures of Escherichia coli by continuous-flow size selection. Journal of General Microbiology 91:188–190
    [Google Scholar]
  7. Lloyd D. 1974; Environmental modification of mitochondrial composition and activities. In The Mitochondria of Micro-organisms pp. 159–250 London: Academic Press.;
    [Google Scholar]
  8. Lloyd D., John L., Edwards C., Chaola A.H. 1975; Synchronous cultures of micro-organisms: large-scale preparation by continous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  9. Mitchison J.M. 1971; The control of division. In The Biology of the Cell Cycle pp. 201–249 Cambridge University Press.;
    [Google Scholar]
  10. Poole R.K. 1975; Respiration in synchronous cultures of a normal and a respiratory-deficient mutant strain of Escherichia coli. Proceedings of the Third European Workshop on the Cell Cycle Edinburgh:September 1975 p. 11:
    [Google Scholar]
  11. Poole R.K. 1977; Fluctuations in buoyant density during the cell cycle of Escherichia coli k12: significance for the preparation of synchronous cultures by age selection. Journal of General Microbiology 98:177–186
    [Google Scholar]
  12. Poole R.K., Haddock B.A. 1975; Dibromothymoquinone: an inhibitor of aerobic electron transport at the level of ubiquinone in Escherichia coli. FEBS Letters 52:13–16
    [Google Scholar]
  13. Poole R.K., Lloyd D. 1974; Changes in respiratory activities during the cell cycle of the fission yeast Schizosaccharomyces pombe 972h growing in the presence of glycerol. Biochemical Journal 144:141–148
    [Google Scholar]
  14. Poole R.K., Lloyd D., Kemp R.B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h. Journal of General Microbiology 77:209–220
    [Google Scholar]
  15. Schairer H.U., Haddock B.A. 1972; β-Galactoside accumulation in a Mg2+-, Ca2+-activated ATPase deficient mutant of E. coli. Biochemical and Biophysical Research Communications 48:544–551
    [Google Scholar]
  16. Scopes A.W., Williamson D.H. 1964; The growth and oxygen uptake of synchronously-dividing cultures of Saccharomyces cerevisiae. Experimental Cell Research 35:361–371
    [Google Scholar]
  17. Smither R. 1975; Use of a Coulter counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli. Journal of Applied Bacteriology 39:137–165
    [Google Scholar]
  18. Stephenson M. 1949; Growth and Nutrition. In Bacterial Metabolism, 3rd. pp. 159–178 London: Longmans Green & Co.;
    [Google Scholar]
  19. Zaritsky A., Pritchard R.H. 1973; Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. Journal of Bacteriology 114:824–837
    [Google Scholar]
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