Transfer and Expression of Pseudomonas Plasmid RP1 in Caulobacter Free

Abstract

Summary: This study demonstrates that the host range of Pseudomonas plasmid RP1 includes the genus Caulobacter was shown to acquire three antibiotic resistance markers located in RP1. A fourth plasmid marker, susceptibility to an RNA bacteriophage, was not expressed, but could be transferred from Caulobacter to The lack of phenotypic expression of the phage marker was manifested by the inability of the phage to adsorb or to produce plaques on Caulobacter transcipients.

Matings of and cv6 were carried out in the presence of bacteriophage 6, a DNA phage that infects and kills only swarmer cells of Caulobacter. No decrease in plasmid transfer in the presence of phage 6 was detected, suggesting that stalked cells, and not swarmer cells, serve as recipients.

Our evidence suggests that transfer of chromosomal segments from Caulobacter may be mediated by plasmid RP1; such segments are not stably maintained.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-99-2-325
1977-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/99/2/mic-99-2-325.html?itemId=/content/journal/micro/10.1099/00221287-99-2-325&mimeType=html&fmt=ahah

References

  1. Adams M.H. 1959 Bacteriophages. New York: Interscience.;
    [Google Scholar]
  2. Anderson E. S. 1968; The ecology of transferable drug resistance in the Enterobacteria. Annual Review of Microbiology 22:131–180
    [Google Scholar]
  3. Anderson E. S., Threlfall E. J. 1970; Change of host range in a resistance factor. Genetical Research 16:207–214
    [Google Scholar]
  4. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. 1972; Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110:529–537
    [Google Scholar]
  5. Jollick J. D. 1972; Differential phage sensitivity of cell types in Caulobacter. Journal of General Virology 16:405–407
    [Google Scholar]
  6. Jollick J. D., Tran T. Q. 1975; Polarity of gene transfer in Caulobacter. Journal of General Microbiology 91:183–187
    [Google Scholar]
  7. Jollick J. D., Wright B. L. 1974; A flagella specific bacteriophage for Caulobacter. Journal of General Virology 22:197–205
    [Google Scholar]
  8. Olsen R. H., Shipley P. 1973; Host range and properties of the Pseudomonas aeruginosa R factor 1822. Journal of Bacteriology 113:772–780
    [Google Scholar]
  9. Poindexter J. 1964; Biological properties and classification of the Caulobacter group. Bacteriological Reviews 28:231–295
    [Google Scholar]
  10. Shapiro L., Maizel J. V.Jr 1973; Synthesis and structure of Caulobacter crescentus flagella. Journal of Bacteriology 113:478–485
    [Google Scholar]
  11. Shapiro L., Agabian-Kesmshian N., Bendis I. 1971; Bacteria differentiation. Science 173:884–892
    [Google Scholar]
  12. Stanisich V. A., Holloway B. W. 1971; Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genetical Research 17:169–172
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-2-325
Loading
/content/journal/micro/10.1099/00221287-99-2-325
Loading

Data & Media loading...

Most cited Most Cited RSS feed