@article{mbs:/content/journal/micro/10.1099/00221287-99-2-243, author = "Indge, Keith and Seaston, Anne and Eddy, A. Alan", title = "The Concentration of Glycine by Saccharomyces uvarum: Role of the Main Vacuole and Conditions Leading to the Explosive Absorption of the Amino Acid", journal= "Microbiology", year = "1977", volume = "99", number = "2", pages = "243-255", doi = "https://doi.org/10.1099/00221287-99-2-243", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-99-2-243", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: At pH 4·3 in the presence of 2% (w/v) glucose and 13 mm-glycine, washed cells of Saccharomyces uvurum took up and retained a maximum of about 1 μmol glycine per mg dry wt. A steady state was reached in which glycine influx was less than 10 % of its initial value, and was largely balanced by the rate of glycine metabolism, efflux being slow. Controlled Jysis of the plasmalemma with cytochrome c indicated that little glycine entered the main vacuole. Preliminary starvation of the yeast for 70 min in the presence of glucose without a nitrogen source led to marked changes: the initial rate of glycine uptake doubled; the amuunt of glycine retained increased to more than 2 μmol mg−1; glycine entered the vacuoles causing them to swell; and many of the cells swelled and burst. The observations indicated that the general amino-acid permease concentrated glycine by a factor of about 5 × 104 at the plasmalemma. The amount of glycine taken up was regulated by both osmotic factors and access to the vacuole.", }