1887

Abstract

SUMMARY: Synchronous cultures obtained by selection at division were used to investigate the occurrence of cold-sensitive stages during the division cycle of (Îind). There are two such stages within the 50 min cycle: one (early) at 10 to 20 min and the other (late) at 40 to 45 min. Similar results were obtained from calculations based both on the age frequency distribution of cells in exponential growth and on the size of the populations which accumulate as a result of a single change of temperature. Times of about 17 and 44 min were found for the early and the late stages, respectively. It is concluded that the two-step doubling of 12 cultures synchronized by a single cold shock is due to two cold-sensitive stages in the division cycle.

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1977-03-01
2024-12-09
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References

  1. Cox J.H., Strack H.B. 1972; Synchronous growth ofEscherichia coli c6oo dependent on a lambdoid prophage.. Virology 50:912–915
    [Google Scholar]
  2. Cummings D.J. 1970; Synchronization of E. coli k12 by Membrane Selection.. Biochemical and Biophysical Research Communications 41:471–476
    [Google Scholar]
  3. Doudney C.O. 1960; Inhibition of nucleic acid synthesis by chloramphenicol in synchronized cultures of Escherichia coli.. Journal of Bacteriology 79:122–124
    [Google Scholar]
  4. Engelberg J. 1964; The decay of synchronization of cell division.. Experimental Cell Research 36:647–662
    [Google Scholar]
  5. Helmstetter C.E. 1967; Rates of DNA synthesis during the division cycle of Escherichia coli B/r.. Journal of Molecular Biology 24:417–427
    [Google Scholar]
  6. Helmstetter C.E., Cummings D.J. 1964; An improved method for the selection of bacterial cells at division.. Biochimica et biophysica acta 82:608–610
    [Google Scholar]
  7. Hotchkiss R.D. 1954; Cyclical behavior in pneumococcal growth and transformability occasioned by environmental changes.. Proceedings of the National Academy of Sciences of the United States of America 40:49–55
    [Google Scholar]
  8. Lark K.G., Maaløe O. 1954; The induction of cellular and nuclear division in Salmonella typhimuriumby means of temperature shifts.. Biochimica et biophysica acta 15:345–356
    [Google Scholar]
  9. Mitchison J.M. 1971 The Biology of the Cell Cycle pp. 51–54 London: Cambridge University Press;
    [Google Scholar]
  10. Perry R.P. 1959; On some properties of growth in a synchronized population of Escherichia coli.. Experimental Cell Research 17:414–419
    [Google Scholar]
  11. Powell E.O. 1955; Some features of the generation times of individual bacteria.. Biometrika 42:16–44
    [Google Scholar]
  12. Scherbaum O.H. 1962; A comparison of synchronized cell division in protozoa.. Journal of Protozoology 9:61–64
    [Google Scholar]
  13. Scherbaum O.H. 1964; Comparison of synchronous and synchronized cell division.. Experimental Cell Research 33:89–98
    [Google Scholar]
  14. Scott D.B.McN., Chu E. 1958; Synchronized division of growing cultures of Escherichia coli.. Experimental Cell Research 14:166–174
    [Google Scholar]
  15. Smith H.S., Pardee A.B. 1970; Accumulation of a protein required for division during the cell cycle of Escherichia coli.. Journal of Bacteriology 101:901–909
    [Google Scholar]
  16. Wolosker H.B.M., Almeida D.F.De 1975; Two-step doubling of synchronous cultures of Escherichia coli k12.. Journal of General Microbiology 88:381–383
    [Google Scholar]
  17. Wolosker H.B.M. 1976 Estudo de algumas propriedades associadas ao processo de sincronizagao de Escherichia coli k12. M.SC. thesis Federal University of Rio de Janeiro, Brazil:
    [Google Scholar]
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