SUMMARY: Intracellular accumulation of zinc by Candida utilisnrrl-y-7634 was mediated by an energy- and temperature-dependent, highly specific process exhibiting saturation kinetics. In zinc-supplemented medium, uptake occurred only during the lag and late-exponential phases; this type of transport did not occur with zinc in bacteria nor with iron in either yeast or bacteria. Cells of C. utilis did not possess a zinc-efflux system; they could reduce their level of intracellular zinc only by dilution of the metal into daughter cells. Zinc-deficient organisms accumulated 12 times more zinc than did cells of the same culture age grown in zinc-supplemented medium. The varied, but experimentally reproducible levels of intracellular zinc that occurred in response to the physiological and environmental parameters had no detectable effects on respiration, rate of growth, total cell yield, or cell viability. Neither the mechanism underlying the cyclic accumulation of zinc nor the function of such behaviour are understood.
AnrakuY., GotoF., KinE.1975; Transport of sugars and amino acids in bacteria. XIII. Mechanism of selective inhibition of the active transport reactions for proline, leucine and succinate by zinc ions.. Journal of Biochemistry, Tokyo 78:149–158
BellengerN., NissenR., WoodT.C., SegelI.H.1968; Specificity and control of choline- O-sulfate transport in filamentous fungi.. Journal of Bacteriology 96:1574–1585
CumminsJ.E., MitchisonJ.M.1967; Adenine uptake and pool formation in the fission yeast Schizo- saccharomyces pombe.. Biochimica et biophysica acta 136:108–120
FaillaM.L.1977; Zinc: functions and transport in microorganisms.. In Microorganisms and Minerals.WeinbergE.D. Edited by New York: Marcel Dekker; in the Press
FisherS., BuxbaumL., TothK., EisenstadtE., SilverS.1973; Regulation of manganese accumulation and exchange in Bacillus subtilis W23.. Journal of Bacteriology 113:1373–1380
GrensonM.1969; The utilization of exogenous pyrimidines and the recycling of uridine-5ʹ-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism.. European Journal of Biochemistry 14:197–204
HunterD.R., SegelI.H.1973; Control of the general amino acid permease of Pencillium chrysogenumby transinhibition and turnover.. Archives of Biochemistry and Biophysics 154:387–399
KasaharaM., AnrakuY.1974; Transport of sugars and amino acids in bacteria. XI. Mechanism of energy coupling reaction for the concentrative uptake of proline by E. coli membrane vesicles.. Journal of Biochemistry, Tokyo 76:977–983
KempnerE.S., MillerJ.H.1972; The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimum culture medium.. Journal of Protozoology 19:343–346
KowarskiS., Blair-StanekC.S., SchachterD.1974; Active transport of zinc and identification of zinc-binding protein in rat jejunal mucosa.. American Journal of Physiology 226:401–407
MorrisonC.E., LichsteinH.C.1976; Regulation of lysine transport by feedback inhibition in Saccharomyces cerevisiae.. Journal of Bacteriology 125:864–871
OhanianceL., ChaixP.1966; Effet inhibiteur de Zn2+ sur la biosynthese induite par l’oxygene des enzymes respiratoires de la levure.. Biochimica et biophysica acta 128:228–238
ParryG.D.R., HaywoodJ.1973; The uptake of65Zn by Dunaliella tertiolecta.. Journal of the Marine Biological Association of the United Kingdom 53:915–922
RichardsM., CousinsR.J.1975; Mammalian zinc homeostasis: requirement for RNA and metallo- thionein synthesis.. Biochemical and Biophysical Research Communications 64:1215–1223
RingK., GrossW., HeinzE.1970; Negative feedback regulation of amino acid transport in Strepto- myces hydrogenans.. Archives of Biochemistry and Biophysics 137:243–252
RosenbergH., MunkN.1969; Transport phenomena associated with the deposition and disappearance of pyrophosphate granules in Tetrahymena pyriformis.. Biochimica et biophysica acta 184:191–197
SchwarzF.J., MatroneG.1975; Methodological studies on the uptake of zinc by 3T3 cells.. Proceedings of the Society for Experimental Biology and Medicine 149:888–892
ScribnerH.E., MogelsonJ., EisenstadtE., SilverS.1975; Regulation of cation transport during bacterial sporulation.. In Spores VI pp. 346–355GerhardtP., CostilowR.N, SadotfH.L. Edited by Washington: American Society for Microbiology;
SkulachevV.P., ChistyakovV.V., JasaitisA.A., SmirnovaE.G.1967; Inhibition of the respiratory chain by zinc ions.. Biochemical and Biophysical Research Communications 26:1–6
WeimbergR.1975; Polyphosphate levels in nongrowing cells of Saccharomyces mellis as determined by magnesium ion and the phenomenon of ‘überkompensation’.. Journal of Bacteriology 121:1122–1130