1887

Abstract

SUMMARY: Intracellular accumulation of zinc by -7634 was mediated by an energy- and temperature-dependent, highly specific process exhibiting saturation kinetics. In zinc-supplemented medium, uptake occurred only during the lag and late-exponential phases; this type of transport did not occur with zinc in bacteria nor with iron in either yeast or bacteria. Cells of did not possess a zinc-efflux system; they could reduce their level of intracellular zinc only by dilution of the metal into daughter cells. Zinc-deficient organisms accumulated 12 times more zinc than did cells of the same culture age grown in zinc-supplemented medium. The varied, but experimentally reproducible levels of intracellular zinc that occurred in response to the physiological and environmental parameters had no detectable effects on respiration, rate of growth, total cell yield, or cell viability. Neither the mechanism underlying the cyclic accumulation of zinc nor the function of such behaviour are understood.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-99-1-85
1977-03-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/99/1/mic-99-1-85.html?itemId=/content/journal/micro/10.1099/00221287-99-1-85&mimeType=html&fmt=ahah

References

  1. Anraku Y., Goto F., Kin E. 1975; Transport of sugars and amino acids in bacteria. XIII. Mechanism of selective inhibition of the active transport reactions for proline, leucine and succinate by zinc ions.. Journal of Biochemistry, Tokyo 78:149–158
    [Google Scholar]
  2. Becker J.M., Lichstein H.C. 1972; Transport overshoot during biotin uptake by Saccharomyces cerevisiae.. Biochimica et biophysica acta 282:409–422
    [Google Scholar]
  3. Bellenger N., Nissen R., Wood T.C., Segel I.H. 1968; Specificity and control of choline- O-sulfate transport in filamentous fungi.. Journal of Bacteriology 96:1574–1585
    [Google Scholar]
  4. Cleland F. 1967; The statistical analysis of enzyme kinetic data.. Advances in Enzymology 29:1–32
    [Google Scholar]
  5. Cummins J.E., Mitchison J.M. 1967; Adenine uptake and pool formation in the fission yeast Schizo- saccharomyces pombe.. Biochimica et biophysica acta 136:108–120
    [Google Scholar]
  6. Failla M.L. 1977; Zinc: functions and transport in microorganisms.. In Microorganisms and Minerals. Weinberg E.D. Edited by New York: Marcel Dekker; in the Press
    [Google Scholar]
  7. Failla M.L., Benedict C.D., Weinberg E.D. 1976; Accumulation and storage of Zn2+ by Candida utilis.. Journal of General Microbiology 94:23–36
    [Google Scholar]
  8. Fisher S., Buxbaum L., Toth K., Eisenstadt E., Silver S. 1973; Regulation of manganese accumulation and exchange in Bacillus subtilis W23.. Journal of Bacteriology 113:1373–1380
    [Google Scholar]
  9. Fiske C.H., Subbarow Y. 1925; The colorimetric determination of phosphorus.. Journal of Biological Chemistry 66:375–400
    [Google Scholar]
  10. Grenson M. 1969; The utilization of exogenous pyrimidines and the recycling of uridine-5ʹ-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism.. European Journal of Biochemistry 14:197–204
    [Google Scholar]
  11. Hunter D.R., Segel I.H. 1973; Control of the general amino acid permease of Pencillium chrysogenumby transinhibition and turnover.. Archives of Biochemistry and Biophysics 154:387–399
    [Google Scholar]
  12. Jones A. 1967; Calcium and phosphorus accumulation in Spirostanum ambiguum.. Journal of Protozoology 14:220–225
    [Google Scholar]
  13. Kadner R.J. 1975; Regulation of methionine transport activity in Escherichia coli.. Journal of Bacteriology 122:110–119
    [Google Scholar]
  14. Kasahara M., Anraku Y. 1974; Transport of sugars and amino acids in bacteria. XI. Mechanism of energy coupling reaction for the concentrative uptake of proline by E. coli membrane vesicles.. Journal of Biochemistry, Tokyo 76:977–983
    [Google Scholar]
  15. Kempner E.S., Miller J.H. 1972; The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimum culture medium.. Journal of Protozoology 19:343–346
    [Google Scholar]
  16. Kleiner D. 1974; The effect of Zn2+ ions on mitochondrial electron transport.. Archives of Biochemistry and Biophysics 165:121–125
    [Google Scholar]
  17. Kowarski S., Blair-Stanek C.S., Schachter D. 1974; Active transport of zinc and identification of zinc-binding protein in rat jejunal mucosa.. American Journal of Physiology 226:401–407
    [Google Scholar]
  18. Lineweaver H., Burk D. 1934; The determination of enzyme dissociation constants.. Journal of the American Chemical Society 56:658–666
    [Google Scholar]
  19. Lomander L. 1965; Influence of calcium and zinc on the growth of Saccharomyces pastorianus.. Physiologia Plantarum 18:153–158
    [Google Scholar]
  20. Morgan R.S., Satillaro R.F. 1972; Zinc in Entamoeba invadens.. Science 176:929–930
    [Google Scholar]
  21. Morrison C.E., Lichstein H.C. 1976; Regulation of lysine transport by feedback inhibition in Saccharomyces cerevisiae.. Journal of Bacteriology 125:864–871
    [Google Scholar]
  22. Ohaniance L., Chaix P. 1966; Effet inhibiteur de Zn2+ sur la biosynthese induite par l’oxygene des enzymes respiratoires de la levure.. Biochimica et biophysica acta 128:228–238
    [Google Scholar]
  23. Okorokov L.A., Kulaev I.S. 1968; On the presence of polymeric form phosphate in mycelium of P. chrysogenumq-176.. Biokhimiya 33:667–679
    [Google Scholar]
  24. Okorokov L.A., Lichko L.P., Kholodenko V.P., Kadomtseva V.M., Petrikevich S.B., Zaichkin E.I., Karimove A.M. 1975; Free and bound magnesium in fungi and yeasts.. Folia microbiologia (Praha) 20:460–466
    [Google Scholar]
  25. Parry G.D.R., Haywood J. 1973; The uptake of65Zn by Dunaliella tertiolecta.. Journal of the Marine Biological Association of the United Kingdom 53:915–922
    [Google Scholar]
  26. Paton W.H.N, Budd K. 1972; Zinc uptake in Neocosmospora vasinfecta.. Journal of General Microbiology 72:173–184
    [Google Scholar]
  27. Ponta H., Broda E. 1970; Mechanismen der Aufnahme von Zink durch Backerhefe.. Planta 95:18–26
    [Google Scholar]
  28. Richards M., Cousins R.J. 1975; Mammalian zinc homeostasis: requirement for RNA and metallo- thionein synthesis.. Biochemical and Biophysical Research Communications 64:1215–1223
    [Google Scholar]
  29. Ring K., Gross W., Heinz E. 1970; Negative feedback regulation of amino acid transport in Strepto- myces hydrogenans.. Archives of Biochemistry and Biophysics 137:243–252
    [Google Scholar]
  30. Rosenberg H., Munk N. 1969; Transport phenomena associated with the deposition and disappearance of pyrophosphate granules in Tetrahymena pyriformis.. Biochimica et biophysica acta 184:191–197
    [Google Scholar]
  31. Rothstein A., Hages A., Jennings D., Hooper D. 1958; The active transport of Mg++ and Mn++ into the yeast cell.. Journal of General Physiology 41:585–594
    [Google Scholar]
  32. Rytha J. 1975; Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae.. Journal of Bacteriology 121:562–570
    [Google Scholar]
  33. Schwarz F.J., Matrone G. 1975; Methodological studies on the uptake of zinc by 3T3 cells.. Proceedings of the Society for Experimental Biology and Medicine 149:888–892
    [Google Scholar]
  34. Scribner H.E., Mogelson J., Eisenstadt E., Silver S. 1975; Regulation of cation transport during bacterial sporulation.. In Spores VI pp. 346–355 Gerhardt P., Costilow R.N, Sadotf H.L. Edited by Washington: American Society for Microbiology;
    [Google Scholar]
  35. Singh A.P., Bragg P.D. 1974; Inhibition of energization of Salmonella typhimurium membrane by zinc ion.. FEBS Letters 40:200–202
    [Google Scholar]
  36. Skulachev V.P., Chistyakov V.V., Jasaitis A.A., Smirnova E.G. 1967; Inhibition of the respiratory chain by zinc ions.. Biochemical and Biophysical Research Communications 26:1–6
    [Google Scholar]
  37. Weimberg R. 1975; Polyphosphate levels in nongrowing cells of Saccharomyces mellis as determined by magnesium ion and the phenomenon of ‘überkompensation’.. Journal of Bacteriology 121:1122–1130
    [Google Scholar]
  38. Weinberg E.D. 1970; Biosynthesis of secondary metabolites: roles of trace metals.. Advances in Microbial Physiology 4:1–44
    [Google Scholar]
  39. Yamamoto K.A., Segel I.H. 1966; The inorganic sulfate transport system of Penicillium chrysogenum.. Archives of Biochemistry and Biophysics 114:523–538
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-1-85
Loading
/content/journal/micro/10.1099/00221287-99-1-85
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error