1887

Abstract

SUMMARY: Chitin synthase (EC 2.4.1.16) from membrane preparations of was characterized and the optimum conditions for enzyme activity were determined. A reaction velocity--substrate concentration plot was sigmoidal, but was hyperbolic in the presence of -acetylglucosamine when the for UDP--acetylglucosamine was 3·1 mmol 1. Chitin synthase activity could be increased sixfold by digestion of enzyme preparations with trypsin for short periods. The trypsin-activated enzyme had altered kinetic and storage properties.

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/content/journal/micro/10.1099/00221287-99-1-69
1977-03-01
2020-01-24
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-1-69
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