1887

Abstract

Summary: Chitin synthase (EC 2.4.1.16) from membrane preparations of was characterized and the optimum conditions for enzyme activity were determined. A reaction velocity-substrate concentration plot was sigmoidal, but was hyperbolic in the presence of -acetylglucosamine when the for UDP--acetylglucosamine was 3·1 mmol 1. Chitin synthase activity could be increased sixfold by digestion of enzyme preparations with trypsin for short periods. The trypsin-activated enzyme had altered kinetic and storage properties.

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/content/journal/micro/10.1099/00221287-99-1-69
1977-03-01
2021-07-27
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