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SUMMARY: Conditions for obtaining colchicine-induced metaphase arrest of the chromosomes of the cellular slime mould Dictyostelium discoideum are described. Using this technique, which increases the mitotic index of exponentially growing amoebae from 1·5 to 11%, it has been demonstrated conclusively that D. discoideum has a haploid chromosome number of seven and that strain ax2 (atcc24397) is not highly aneuploid as has recently been suggested. Each of the seven chromosomes can be identified by a characteristic banding pattern seen after staining with Giemsa.
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