1887

Abstract

SUMMARY: Conditions for obtaining colchicine-induced metaphase arrest of the chromosomes of the cellular slime mould are described. Using this technique, which increases the mitotic index of exponentially growing amoebae from 1·5 to 11%, it has been demonstrated conclusively that has a haploid chromosome number of seven and that strain (24397) is not highly aneuploid as has recently been suggested. Each of the seven chromosomes can be identified by a characteristic banding pattern seen after staining with Giemsa.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-99-1-201
1977-03-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/99/1/mic-99-1-201.html?itemId=/content/journal/micro/10.1099/00221287-99-1-201&mimeType=html&fmt=ahah

References

  1. Bonner J.T., Frascella E.B. 1952; Mitotic activity in relation to differentiation in the slime mold Dictyostelium discoideum.. Journal of Experimental Zoology 121:561–571
    [Google Scholar]
  2. Brody T., Williams K.L. 1974; Cytological analysis of the parasexual cycle in Dictyostelium discoideum.. Journal of General Microbiology 82:371–383
    [Google Scholar]
  3. Fukui Y. 1974; Cell fusion as a prototype of sexual reproduction: parasexual cycle in the cellular slime molds.. Symposium for Cell Biology 26:47–57
    [Google Scholar]
  4. Fukui Y., Miyake Y. 1975; Parasexual hybridization in cellular slime molds. I. Appearance of hybrid clones at high frequency in a short period and its relation to cell fusion in Dictyostelium discoideum.. Cell Structure and Function 1:23–31
    [Google Scholar]
  5. Huffman D.M., Olive L.S. 1964; Engulfment and anastomosis in the cellular slime molds (Acrasiales).. American Journal of Botany 51:465–471
    [Google Scholar]
  6. Moens P.B. 1976; Spindle and kinetochore morphology of Dictyostelium discoideum.. Journal of Cell Biology 68:113–122
    [Google Scholar]
  7. Muroyama T., Hashimoto Y., Sameshima M., Yamada T. 1975; The karyotype of the cellular slime molds.. Chromosome Information Service 19:32–33
    [Google Scholar]
  8. Sackin M.J., Ashworth J.M. 1969; An analysis of the distribution of volumes amongst spores of the cellular slime mouldDictyostelium discoideum.. Journal of General Microbiology 59:275–284
    [Google Scholar]
  9. Sinha U., Ashworth J.M. 1969; Evidence for the existence of elements of a parasexual cycle in the cellular slime mouldDictyostelium discoideum.. Proceedings of the Royal Society of London B 173:531–540
    [Google Scholar]
  10. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development.. In Methods in Cell Physiology 2 pp. 397–410 Prescott D. Edited by New York:: Academic Press.;
    [Google Scholar]
  11. Sussman M., Sussman R.R. 1962; Ploidal inheritance in Dictyostelium discoideum: stable haploid, stable diploid and metastable strains.. Journal of General Microbiology 28:417–429
    [Google Scholar]
  12. Sussman R.R. 1961; A method for staining the chromosomes of Dictyostelium discoideummyxamoebae in the vegetative stage.. Experimental Cell Research 24:154–155
    [Google Scholar]
  13. Watts D.J., Ashworth J.M. 1970; Growth of myxamoebae of the cellular slime mouldDictyostelium discoideum in axenic culture.. Biochemical Journal 119:171–174
    [Google Scholar]
  14. Wilson C.M. 1952; Sexuality in the Acrasiales.. Proceedings of the National Academy of Sciences of the United States of America 38:659–662
    [Google Scholar]
  15. Wilson C.M. 1953; Cytological study of the life cycle of Dictyostelium.. American Journal of Botany 40:714–718
    [Google Scholar]
  16. Wilson C.M., Ross I.K. 1957; Further cytological studies in the Acrasiales.. American Journal of Botany 44:345–350
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-1-201
Loading
/content/journal/micro/10.1099/00221287-99-1-201
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error