%0 Journal Article %A Kennedy, Christina %A Postgate, J. R. %T Expression of Klebsiella pneumoniae Nitrogen Fixation Genes in Nitrate Reductase Mutants of Escherichia coli %D 1977 %J Microbiology, %V 98 %N 2 %P 551-557 %@ 1465-2080 %R https://doi.org/10.1099/00221287-98-2-551 %I Microbiology Society, %X Summary: Nitrate reductase (nar) A, B and E mutants of Escherichia coli with plasmids carrying Klebsiella pneumoniae nitrogen fixation (nif) genes reduced acetylene independently of added molybdate, but nar D mutants showed pleiotropic dependence on the concentration of added molybdate for expression of both nar and nif. No complementation of nar mutations by nif occurred; nitrite but not nitrate repressed nif in nar hosts. Derepression of nif occurred in molybdenum-deficient nar D (nif) strains since nitrogenase peptides were present. nifB mutants, thought to have a lesion in the pathway of molybdenum to nitrogenase, as well as nif deletion mutants, had normal nitrate reductase activity. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-98-2-551