1887

Abstract

Summary: Esterases of 42 strains of and Citrobacter were analysed by horizontal slab electrophoresis in polyacrylamide-agarose gel using several synthetic substrates. On the basis of esterase zymograms a distinctive pattern was established for each of the three species. was characterized by two major bands: one hydrolysing acetate esters but not butyrate esters; and the other hydrolysing -naphthyl acetate and reacting weakly with -naphtyl butyrate and -napthyl acetate. showed one prominent band that hydrolysed -naphthyl esters and reacted weakly with -naphthyl esters. Citrobacter strains showed one major band that hydrolysed -naphthyl esters and appeared slightly more active towards -naphthyl esters than that of Considerable variations in electrophoretic mobility were observed among Citrobacter strains. was less variable. In addition, one minor anodal band reacting with -naphthyl acetate was observed in both and Citrobacter.

The relative molecular sizes of the major esterase bands were determined by disc electrophoresis with gels of different acrylamide concentrations. The molecular size of the major band of Citrobacter appeared to be smaller than that of the corresponding esterase band of

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1977-02-01
2021-10-16
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References

  1. Crosa J. H., Steigerwalt A. G., Fanning G. R., Brenner D. J. 1974; Polynucleotide sequence divergence in the genus Citrobacter. Journal of General Microbiology 83:271–282
    [Google Scholar]
  2. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  3. Goullet Ph. 1973; An esterase zymogram of Escherichia coli. Journal of General Microbiology 77:27–35
    [Google Scholar]
  4. Goullet Ph. 1975; Esterase zymograms of Proteus and Providencia. Journal of General Microbiology 87:97–106
    [Google Scholar]
  5. Hedrick J. L., Smith A. J. 1968; Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Archives of Biochemistry and Biophysics 126:155–164
    [Google Scholar]
  6. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
    [Google Scholar]
  7. Richard C., Brisou B., Lioult J. 1972; Etude taxonomique de ‘Levinea’, nouveau genre de la famille des Entérobactéries. Annales de l’Institut Pasteur 122:1137–1146
    [Google Scholar]
  8. Uriel J. 1961; Caractérisation des cholinestérases et d’autres estérases carboxyliques après électrophorèse et immunoélectrophorèse en gélose (application a 1’étude des estérases du sérum humain normal). Annales de l’Institut Pasteur 101:104–119
    [Google Scholar]
  9. Uriel J. 1966; Méthode d’électrophorèse dans des gels d’acrylamide-agarose. Bulletin de la Société de chimie biologique 48:969–982
    [Google Scholar]
  10. Young V. M., Kenton D. M., Hobbs B. J., Moody M. R. 1971; Levinea, a new genus of the family Enterobacteriaceae. International Journal of Systematic Bacteriology 21:58–63
    [Google Scholar]
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