1887

Abstract

Summary: Eleven mutants resistant to a toxic analogue of asparagine, aspartic hydroxamate, have been isolated; they are allelic and map at the locus. These mutations result in low or non-detectable asparaginase activity. mutations are recessive for asparaginase activity and aspartic hydroxamate resistance. The locus is in linkage group VIII and is loosely linked with and Asparaginase activity was measured the by formation of aspartic hydroxamate from asparagine and hydroxylamine. The values of asparaginase for asparagine and hydroxylamine are 0·6 and 8·3 mM respectively. Minimum asparaginase activity is present in cells grown on ammonium or glutamine. Maximum asparaginase activity is present in wild-type cells grown on ammonium and then held in nitrogen-free medium for 3 h. Derepression from this ammonium repression requires protein synthesis. A number of different types of ammonium-repressed and of ammonium-derepressed mutants have abnormal regulation of asparaginase activity.

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1977-02-01
2021-07-24
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References

  1. Adelberg E.A., Mandel M., Chen G.C.C. 1965; Optimal conditions for mutagenesis by N-methyl-N-nitro-iV-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Arst H.N., Cove D.J. 1969; Methylammonium resistance in Aspergillus nidulans. Journal of Bacteriology 98:1284–1293
    [Google Scholar]
  3. Arst H.N., Page M.M. 1973; Mutants of Aspergillus nidulans altered in the transport of methyl-ammonium and ammonium. Molecular and General Genetics 121:239–245
    [Google Scholar]
  4. Capizzi R.L., Bertino J.R., Handschumacher R.E. 1970; l-Asparaginase. Annual Review of Medicine 21:433–444
    [Google Scholar]
  5. Clutterbuck A.J. 1968; Cell volume per nucleus in haploid and diploid strains of Aspergillus nidulans. Journal of General Microbiology 55:291–299
    [Google Scholar]
  6. Clutterbuck A.J. 1974; Aspergillus nidulans. In Handbook of Genetics 1 pp. 447–510 King R.C. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Clutterbuck A.J., Cove D.J. 1974; Linkage map of Aspergillus nidulans. In Handbook of Microbiology. IV Microbial Metabolism, Genetics and Immunology pp. 665–676 Laskin A.I., Lechevalier H.A. Edited by Cleveland, Ohio: C.R.C. Press;
    [Google Scholar]
  8. Cohen B.L. 1972; Ammonium repression of extracellular protease in Aspergillus nidulans. Journal of General Microbiology 71:293–299
    [Google Scholar]
  9. Cove D.J. 1966; The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et biophysica acta 113:51–56
    [Google Scholar]
  10. De-Angeli L.C., Pocochani F., Russi S., Tonolo A., Zurita V.E. 1970; Effect of l-asparaginase from Aspergillus terreus on ascites sarcoma in the rat. Nature; London: 225549–550
    [Google Scholar]
  11. Degroot N., Lichtenstein N. 1960a; The action of mammalian liver enzyme preparations on asparagine and asparagine derivatives. Biochimica et biophysica acta 40:92–98
    [Google Scholar]
  12. DeGroot N., Lichtenstein N. 1960b; The action of Pseudomonas fluorescens extracts on asparagine and asparagine derivatives. Biochimica et biophysica acta 40:99–110
    [Google Scholar]
  13. Dunn E., Pateman J.A. 1972; Urea and thiourea uptake in Aspergillus nidulans. Heredity 29:129
    [Google Scholar]
  14. Ehrman M., Cedar H., Schwartz J.H. 1971; l-Asparaginase II of Escherichia coli. Journal of Biological Chemistry 246:88–94
    [Google Scholar]
  15. Hynes M.J. 1970; Induction and repression of amidase enzymes in Aspergillus nidulans. Journal of Bacteriology 103:482–487
    [Google Scholar]
  16. Hynes M.J., Pateman J.A. 1970; The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans, I & II. Molecular and General Genetics 108:97–116
    [Google Scholar]
  17. Imada A., Igarasi S., Nakahama K., Isono M. 1973; Asparaginase and glutaminase activities of micro-organisms. Journal of General Microbiology 76:85–99
    [Google Scholar]
  18. Kidd J.C. 1953; Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum (I and II). Journal of Experimental Medicine 98:565–606
    [Google Scholar]
  19. Kinghorn J.R., Pateman J.A. 1972; Regulation of glutamate transport in Aspergillus nidulans. Heredity 29:128
    [Google Scholar]
  20. Kinghorn J.R., Pateman J.A. 1973; A new class of mutants affecting ammonium regulation and methylammonium resistance in Aspergillus nidulans. Heredity 31:477–478
    [Google Scholar]
  21. Kinghorn J.R., Pateman J.A. 1975; Studies of partially repressed mutants at the tamA and areA loci in Aspergillus nidulans. Molecular and General Genetics 140:137–147
    [Google Scholar]
  22. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Mccully K.S., Forbes E. 1965; The use of p-fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6:352–359
    [Google Scholar]
  24. Mashburn L.T., Wriston J.C.Jr 1963; Tumour inhibitory effect of l-asparaginase. Biochemical and Biophysical Research Communications 12:50–55
    [Google Scholar]
  25. Mashburn L.T., Wriston J.C.Jr 1964; Tumour inhibitory effect of l-asparaginase from Escherichia coli. Archives of Biochemistry and Biophysics 105:451–452
    [Google Scholar]
  26. Pateman J.A., Cove D.J. 1967; Regulation of nitrate reduction in Aspergillus nidulans. Nature; London: 2151734–1737
    [Google Scholar]
  27. Pateman J.A., Kinghorn J.R. 1975; The tamA locus in Aspergillus nidulans. Heredity 35:27
    [Google Scholar]
  28. Pateman J.A., Kinghorn J.R. 1976; Nitrogen metabolism. In Filamentous Fungi 2 pp. 159–237 Smith J.E., Berry D. Edited by London: Edward Arnold;
    [Google Scholar]
  29. Pateman J.A., Kinghorn J.R., Dunn E., Forbes E. 1973; Ammonium regulation in Aspergillus nidulans. Journal of Bacteriology 114:943–950
    [Google Scholar]
  30. Pateman J.A., Kinghorn J.R., Dunn E. 1974; Regulatory aspects of l-glutamate transport in Aspergillus nidulans. Journal of Bacteriology 119:534–542
    [Google Scholar]
  31. Pontecorvo G., Roper J.A., Hemmons L.M., Macdonald K.D., Bufton A.W.J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  32. Scazzocchio C., Darlington A.J. 1968; The induction and repression of the enzymes of purine breakdown in Aspergillus nidulans. Biochimica et biophysica acta 166:551–568
    [Google Scholar]
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