1887

Abstract

Summary: A3(2) and 66, which lack chloramphenicol acetyltransferase, gave rise to chloramphenicol-sensitive (Cml) variants spontaneously at frequencies of 0·5 to 2%. The fertility type of in respect of the scP1 plasmid (SCP1, SCP1 or NF) had no effect on chloramphenicol sensitivity or on the frequency at which Cml variants arose.

Cml isolates spontaneously reverted to Cml at frequencies one to three orders of magnitude lower than the frequency with which Cml strains arose from Cml Cml revertants obtained spontaneously from Cml clones again produced Cml isolates at the normal frequency of several per cent. Therefore, Cml and Cml are reversible phenotypes.

In crosses between marked Cml and Cml strains, transfer of chloramphenicol resistance into the sensitive strain apparently occurred independently of chromosomal recombination. Mapping experiments excluded the possibility that segregation of a chromosomal locus determines Cml versus Cml phenotype. In crosses between scP1 strains, fertility was not significantly different in Cml × Cml, Cml × Cml and Cml × Cml combinations.

Covalently closed circular DNA from Cml and Cml strains of was indistinguishable in molecular weight and restriction endonuclease cleavage pattern.

It is suggested that chloramphenicol resistance in 3(2) is affected by some kind of transposable genetic element that may be capable of extra-chromosomal existence.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-98-2-453
1977-02-01
2021-05-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/98/2/mic-98-2-453.html?itemId=/content/journal/micro/10.1099/00221287-98-2-453&mimeType=html&fmt=ahah

References

  1. Benveniste R., Davies J. 1973; Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proceedings of the National Academy of sciences of the United States of America 70:2276–2280
    [Google Scholar]
  2. Bérdy J. 1974; Recent developments of antibiotic research and classification of antibiotics according to chemical structure. Advances in Applied Microbiology 18:309–406
    [Google Scholar]
  3. Boronin A. M., Sadovnikova I. G. 1972; Elimination by acridine dyes of oxytetracycline resistance in Actinomyces rimosus. Genetika 8:174–176
    [Google Scholar]
  4. Chater K. F., Wilde L. C. 1976; Restriction of a bacteriophage of Streptomyces albusg involving endonuclease Sail. Journal of Bacteriology 128:644–650
    [Google Scholar]
  5. Cohen S. N., Kopecko D. J. 1976; Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA insertion sequences. Federation Proceedings 35:2031–2036
    [Google Scholar]
  6. Freeman R. F. 1976 Studies of antibiotic resistance and production in Streptomyces. Ph. D. thesis; University of East Anglia, Norwich.:
    [Google Scholar]
  7. Freeman R. F., Bibb M. J., Hopwood D. A. 1976; CAT-independent extra-chromosomal chloram-phenicol resistance in Streptomyces coelicolor a3(2). Proceedings of the Society for General Microbiology 3:183–184
    [Google Scholar]
  8. Harold R. J., Hopwood D. A. 1970; Ultraviolet-sensitive mutants of Streptomyces coelicolor. I. Phenotypic characterisation. Mutation Research 10:427–438
    [Google Scholar]
  9. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  10. Hopwood D. A., Chater K. F. 1974 Streptomyces coelicolor. In Handbook of Genetics 1 pp. 237–255 King R. C. Edited by New York and London:: Plenum Press.;
    [Google Scholar]
  11. Hopwood D. A., Wright H. M. 1976; Interactions of the plasmid SCP1 with the chromosome of Streptomyces coelicolor a3(2). In Second International Symposium on the Genetics of Industrial Microorganisms pp. 607–619 Macdonald K. D. Edited by London and New York:: Academic Press.:
    [Google Scholar]
  12. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  13. Humphreys G. O., Willshaw G. A., Anderson E. S. 1975; A simple method for the preparation of large quantities of pure plasmid DNA. Biochimica et biophysica acta 383:457–473
    [Google Scholar]
  14. Kirby R., Wright L. F., Hopwood D. A. 1975; Plasmid determined antibiotic production and resistance in Streptomyces coelicolor. Nature; London: 254265–267
    [Google Scholar]
  15. Kleckner N., Chan R. K., Tye B.-K., Botstein D. 1975; Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. Journal of Molecular Biology 97:561–575
    [Google Scholar]
  16. Kupersztock-Portnoy Y. M., Miklos G. L.G., Helinski D. R. 1974; Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K. Journal of Bacteriology 120:545–548
    [Google Scholar]
  17. Lomovskaya N. D., Mkrtumian N. M., Gostimskaya N. L., Danilenko V. N. 1972; Characterisation of temperate actinophage ϕC31 isolated from Streptomyces coelicolor a3(2). Journal of Virology 9:258–262
    [Google Scholar]
  18. Ohtsubo H., Ohtsubo E. 1976; Isolation of inverted repeat sequences, including ISi, IS2, and IS3, in Escherichia coli plasmids. Proceedings of the National Academy of sciences of the United States of America 73:2316–2320
    [Google Scholar]
  19. Okanishi M., Ohta T., Umezawa H. 1970; Possible control of formation of aerial mycelium and antibiotic production in Streptomyces by episomic factors. Journal of Antiobitics (Japan) 23:45–47
    [Google Scholar]
  20. schrempf H., Bujard H., Hopwood D. A., Goebel W. 1975; Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor a3(2). Journal of Bacteriology 121:416–421
    [Google Scholar]
  21. Shaw W. V. 1974; Genetics and enzymology of chloramphenicol resistance. Biochemical Society Transactions 2:834–838
    [Google Scholar]
  22. Shaw W. V., Hopwood D. A. 1976; Chloramphenicol acetylation in Streptomyces. Journal of General Microbiology 94:159–166
    [Google Scholar]
  23. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor a3(2): plasmid involvement in the interconversion of uf and if strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
  24. Wright L. F., Hopwood D. A. 1976; Identification of the antibiotic determined by the SCP1 plasmid of Streptomyces coelicolor a3(2). Journal of General Microbiology 95:96–106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-98-2-453
Loading
/content/journal/micro/10.1099/00221287-98-2-453
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error