1887

Abstract

SUMMARY: Mutants with defective lipopolysaccharides (LPSs) were isolated from PACIR (Habs serogroup 3) by selection for resistance to aeruginocin from P16. Carbenicillin-sensitive mutants were isolated from PACI but not all had defective LPSs. Rough colonial morphology and resistance to bacteriophage 119X appeared to be independent of LPS composition.

The LPSs from five mutants were analysed and compared with that of the parent strain. Separation of partially-degraded polysaccharides from LPS from PACI on Sephadex G75 yielded two different high molecular weight fractions and a phosphorylated low molecular weight fraction (L). The mutant LPSs lacked most or all of the high molecular weight fractions but retained some low molecular weight material. That from PACI and two of the mutants was separated by elution from Biogel P6 into two fractions. One, L2, was the core polysaccharide while the other, LI, contained short antigenic side-chains attached to the core like the semi-rough (SR) LPSs of the Enterobacteriaceae. The two mutants which gave the LI fraction reacted with Habs 3 and PACI antisera as did the parent strain. The other three mutants were unreactiye and their LPSs contained core components only. One appeared to have a complete core while the other two lacked rhamnose and rhamnose plus glucose respectively. Thus there may be four types of LPS in PACI: one contains unsubstituted core polysaccharide and yields L2 on acid hydrolysis, another has short antigenic side-chains of the SR type and yields the LI fraction, while the two high molecular weight fractions are derived from core polysaccharides with different side-chains.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-98-2-387
1977-02-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/98/2/mic-98-2-387.html?itemId=/content/journal/micro/10.1099/00221287-98-2-387&mimeType=html&fmt=ahah

References

  1. Bartlett G.R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  2. Brammar W.J., Clarke P.H., Skinner A.J. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system. Journal of General Microbiology 47:87–102
    [Google Scholar]
  3. Brown P.R., Clarke P.H. 1972; Amino acid substitution in an amidase produced by an acetanilide mutant of Pseudomonas aeruginosa. Journal of General Microbiology 70:287–298
    [Google Scholar]
  4. Chester I.R., Meadow P.M. 1975; Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa. European Journal of Biochemistry 58:273–282
    [Google Scholar]
  5. Chester I.R., Meadow P.M. 1973; The relationship between the O-antigenic lipopoly- saccharides and serological specificity in strains of Pseudomonas aeruginosa of different O-serotypes. Journal of General Microbiology 78:305–318
    [Google Scholar]
  6. Clarkson C.E., Meadow P.M. 1971; Diaminopimelic acid and lysine auxotrophs of Pseudomonas aeruginosa 8602. Journal of General Microbiology 66:161–169
    [Google Scholar]
  7. Drewry D.T., Symes K.C., Gray G.W., Wilkinson S.G. 1975; Studies of polysaccharide fractions from the lipopolysaccharide of Pseudomonas aeruginosa NCTC1999. Biochemical Journal 149:93–106
    [Google Scholar]
  8. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  9. Fensom A.H., Meadow P.M. 1970; Evidence for two regions in the polysaccharide moiety of the lipopolysaccharide of Pseudomonas aeruginosa 8602. FEBS Letters 9:81–84
    [Google Scholar]
  10. Govan J.R.W. 1974; Studies on the pyocins of Pseudomonas aeruginosa: morphology and mode of action of contractile pyocins. Journal of General Microbiology 80:1–15
    [Google Scholar]
  11. Gustafsson P., Nordstrom K., Normark S. 1973; Outer penetration barrier of Escherichia coli K12: kinetics of the uptake of gentian violet by wild type and envelope mutants. Journal of Bacteriology 116:893–900
    [Google Scholar]
  12. Habs I. 1957; Untersuchungen über die O-antigene von Pseudomonas aeruginosa. Zeitschrift für Hygiene und lnfektionskrankheiten 144:218–228
    [Google Scholar]
  13. Hoare D.S., Work E. 1955; The stereoisomers of α,e-diaminopimelic acid: their distribution in nature and behaviour towards certain enzyme preparations. Biochemical Journal 61:562–568
    [Google Scholar]
  14. Holloway B.W., Krishnapillai V. 1974; Bacteriophages and bacteriocins. In Genetics and Biochemistry of Pseudomonas pp. 99–132 Clarke P.H., Richmond M.H. Edited by London:: John Wiley.;
    [Google Scholar]
  15. Ikeda K., Egami F. 1973; Lipopolysaccharides of Pseudomonas aeruginosa with special reference to pyocin R receptor activity. Journal of General and Applied Microbiology 19:115–128
    [Google Scholar]
  16. Ito S., Kageyama M., Egami F. 1970; Isolation and characterisation of pyocins from several strains of Pseudomonas aeruginosa. Journal of General and Applied Microbiology 16:205–214
    [Google Scholar]
  17. Kageyama M. 1975; Bacteriocins and bacteriophages in Pseudomonas aeruginosa. In Microbial Drug Resistance pp. 291–305 Mitsuhashi S., Hashimoto H. Edited by Tokyo:: University of Tokyo Press.;
    [Google Scholar]
  18. Koval S.F., Meadow P.M. 1975a; Lipopolysaccharide-defective mutants of Pseudomonas aeruginosa PACI. Proceedings of the Society for General Microbiology 3:53
    [Google Scholar]
  19. Koval S.F., Meadow P.M. 1975b; The relationship between aminosugars in the lipopolysaccharide, serotype and aeruginocin sensitivity in strains of Pseudomonas aeruginosa. Journal of General Microbiology 91:437–440
    [Google Scholar]
  20. Lüderitz O., Westphal O., Staub A.M., Nikaido H. 1971; Isolation and immunological characterisation of bacterial lipopolysaccharides. In Microbial Toxins IV pp. 145–233 Wein-baum G., Kadis S., Ajl S.J. Edited by London and New York:: Academic Press.;
    [Google Scholar]
  21. Martin D. 1971 Variation in Pseudomonas aeruginosa. Ph.D. thesis; University of London.:
    [Google Scholar]
  22. Meadow P.M. 1975; Wall and membrane structure in the genus Pseudomonas. In Genetics and Biochemistry of Pseudomonas pp. 67–98 Clarke P.H., Richmond M.H. Edited by London:: John Wiley.;
    [Google Scholar]
  23. Naide Y., Nikaido H., Mäkelä P.H., Wilkinson R.G., Stocker B.A.D. 1965; Semi-rough strains of Salmonella. Proceedings of the National Academy of Sciences of the United States of America 53:147–153
    [Google Scholar]
  24. Nowotny A. 1971; Chemical and biological heterogeneity of endotoxins. In Microbial Toxins IV pp. 309–329 Weinbaum G., Kadis S., Ajl S.J. Edited by London and New York:: Academic Press.;
    [Google Scholar]
  25. Rondle C.G.M., Morgan W.T.J. 1955; Determination of glucosamine and galactosamine. Biochemical Journal 61:586–589
    [Google Scholar]
  26. Sanderson K.E., Macalister T., Costerton J.W. 1974; Permeability of lipopolysaccharide-deficient (rough) mutants of Salmonella typhimurium to antibiotics, lysozyme and other agents. Canadian Journal of Microbiology 20:1135–1145
    [Google Scholar]
  27. Sanderson K.E., Van Wyngaarden J., Lüderitz O., Stocker B.A.D. 1974; Rough mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide core. Canadian Journal of Microbiology 20:1127–1134
    [Google Scholar]
  28. Schlect S., Westphal O. 1968; Antibiotica-Empfindlichkeit bei S- und R-Formen von Salmonella minnesota. Naturwissenschaften 10:494–495
    [Google Scholar]
  29. Suzuki N. 1974; Correlation between pyocin sensitivity and 2-aminosugar composition of Pseudomonas aeruginosa. FEBS Letters 48:301–305
    [Google Scholar]
  30. Tamaki S., Matsuhashi M. 1973; Increase in sensitivity to antibiotics and lysozyme on deletion of lipopolysaccharides in Escherichia coli strains. Journal of Bacteriology 114:453–454
    [Google Scholar]
  31. Tamaki S., Sato T., Matsuhashi M. 1971; Role of lipopolysaccharides in antibiotic resistance and bacteriophage absorption of Escherichia coli K12. Journal of Bacteriology 105:968–975
    [Google Scholar]
  32. Wahba A.H., Darrell J.H. 1965; The identification of atypical strains of Pseudomonas aeruginosa. Journal of General Microbiology 38:329–342
    [Google Scholar]
  33. Wilkinson S.G., Galbraith L. 1975; Studies of lipopolysaccharide from Pseudomonas aeruginosa. European Journal of Biochemistry 52:331–343
    [Google Scholar]
  34. Wilkinson R.G., Gemski P., Stocker B.A.D. 1972; Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. Journal of General Microbiology 70:527–554
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-98-2-387
Loading
/content/journal/micro/10.1099/00221287-98-2-387
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error