1887

Abstract

SUMMARY: Synthesis of glutamine synthetase (GS) in anaerobic batch cultures of was repressed when excess NH was available, but derepressed during growth with a poor nitrogen source. In wild-type bacteria there was only a weak inverse correlation between the activities of GS and glutamate dehydrogenase (GDH) during growth in various media. No positive correlations were found between the activities of GS and nitrite reductase, or between GS and cytochrome : both of these proteins were synthesized normally by mutants that contained no active GS. Although activities of GS and GDH were low in two mutants that are unable to synthesize cytochrome or reduce nitrite because of defects in the gene, the defect was separated from the GS and GDH defects by transduction with bacteriophage PI. Attempts to show that catabolite repression of proline oxidase synthesis could be relieved during NH+ starvation also failed. It is, therefore, unlikely that nitrite reduction or proline oxidation by are under positive control by GS protein. The regulation of the synthesis of enzymes for the utilization of secondary nitrogen sources in is, therefore, different from that in , but is similar to that in

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1977-02-01
2021-07-31
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