1887

Abstract

SUMMARY: Myxamoebae of strain Ax-2 were adapted to growth in a medium containing B vitamins, a vitamin-free tryptic digest of casein, soluble starch and inorganic salts. Omission of vitamins from this medium confirmed that -biotin, thiamin, folate and a-lipoate are essential for myxamoebal growth and showed that riboflavin or flavin mononucleotide is also required. No requirement for other vitamins or growth factors could be detected. A defined medium was developed by replacement of the tryptic digest of casein with a mixture of 20 -amino acids and addition of Tween 80. Tween 80 caused starch precipitation in the medium and this seemed essential for stimulating growth of the myxamoebae.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-98-2-355
1977-02-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/98/2/mic-98-2-355.html?itemId=/content/journal/micro/10.1099/00221287-98-2-355&mimeType=html&fmt=ahah

References

  1. Holst-Sørensen H., Rasmussen L. 1971; Growth promoting effects of particulate material in cultures of Acanthamoeba. Comptes rendus des travaux du Laboratoire Carlsberg 38:163–170
    [Google Scholar]
  2. Rasmussen L., Kludt T.R. 1970; Particulate material as a prerequisite for rapid cell multiplication in Tetrahymena cultures. Experimental Cell Research 59:457–463
    [Google Scholar]
  3. Roberts E.C., Snell E.E. 1944; An improved medium for microbiological assays with Lactobacillus casei. Journal of Biological Chemistry 163:499–509
    [Google Scholar]
  4. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. In Methods in Cell Physiology 2 pp. 397–410 Prescott D.M. New York and London:: Academic Press.;
    [Google Scholar]
  5. Watts D.J., Ashworth J.M. 1970; Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
  6. Watts D.J., Guest J.R. 1975; Studies on the vitamin nutrition of the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 86:333–342
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-98-2-355
Loading
/content/journal/micro/10.1099/00221287-98-2-355
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error