%0 Journal Article %A Kirby, R. %A Hopwood, D. A. %T Genetic Determination of Methylenomycin Synthesis by the SCP1 Plasmid of Streptomyces coelicolora3(2) %D 1977 %J Microbiology, %V 98 %N 1 %P 239-252 %@ 1465-2080 %R https://doi.org/10.1099/00221287-98-1-239 %I Microbiology Society, %X Summary: Evidence is presented that genes determining the pathway of methylenomycin A synthesis are carried on the SCP1 plasmid. All 16 mutations (mmy) leading to lack of antibiotic synthesis were SCP1-linked. Phenotypic classification, by co-synthesis and other criteria, suggested that they fell into at least five classes. When the wild-type SCP1 plasmid was transferred to Streptomyces lividans or Streptomyces parvulus, material that was chromatographically and biologically indistinguishable from methylenomycin A was produced. Recombination between some pairs of mmy mutations was detected. In crosses of mmy mutants of NF (integrated SCP1 donor) strains with SCP1−, a very high frequency of chromosomal recombination occurred; thus methylenomycin production appears not to be an important cause of the ultra-fertility normally associated with nf × SCP1− crosses. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-98-1-239