SUMMARY: A technique is described by which both oxygen and nitrate (or nitrite or chlorate) levels were continuously monitored during bacterial respiration. and oxidizing succinate rapidly ceased to reduce nitrate when oxygen was available, and equally rapidly commenced nitrate reduction when all the oxygen had been consumed. By contrast, membrane vesicles isolated from reduced oxygen and nitrate simultaneously. The respiratory nitrate reductase in intact cells of appeared to be inaccessible to chlorate present in the reaction medium, and it is suggested that the nitrate reductase is orientated on the plasma membrane so that nitrate gains access from the inner (cytosolic) face.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error