RT Journal Article SR Electronic(1) A1 Davidson, I. W. A1 Lawson, C. J. A1 Sutherland, I. W.YR 1977 T1 An Alginate Lyase from Azotobacter vinelandii Phage JF Microbiology, VO 98 IS 1 SP 223 OP 229 DO https://doi.org/10.1099/00221287-98-1-223 PB Microbiology Society, SN 1465-2080, AB Summary: The alginate depolymerase associated with bacteriophage infection of Azotobacter vinelandii has been used in the analysis of sodium alginate. The enzyme degraded the polysaccharide to a series of oligouronides each containing a terminal 4-deoxy-α-l-erythro-hex-4-enopyranuronosyl residue. Analysis of these oligouronides, together with kinetic information, indicated that the enzyme was specific for mannuronic acid-containing regions of the polyuronide. The specificity of the enzyme made it possible to determine the primary structure of the macromolecule. The phage-induced enzyme was shown to be distinct from the alginate lyase elaborated by the host organisms by its pH optimum, molecular weight, Michaelis constant and stability., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-98-1-223