1887

Abstract

Summary: The growth rate of was increased by adding a mixture of amino acids to cultures containing proline as the sole nitrogen source. The transition from balanced growth in the basal medium (doubling time 4 h) to balanced growth in the enriched medium (doubling time 2 h) took about 2·5 h. The rate of RNA accumulation increased soon after the enrichment to almost its final value. This increase began after a short lag of 10 to 15 min, therefore synthesis of new RNA polymerase molecules may be required before stable RNA production can increase. The different stable RNA species were not stimulated at different times after the enrichment, but all increased continuously throughout the transition. The rRNA species accumulated in a co-ordinate fashion at a rate faster than the rate of tRNA accumulation.

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/content/journal/micro/10.1099/00221287-98-1-215
1977-01-01
2021-10-27
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References

  1. Adman R., Schultz L.D., Hall B.D. 1972; Transcription in yeast: separation and properties of multiple RNA polymerases.. Proceedings of the National Academy of Sciences of the United States of America 69:1702–1706
    [Google Scholar]
  2. Boehlke K.W., Friesen J.D. 1975; Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate.. Journal of Bacteriology 121:429–433
    [Google Scholar]
  3. Bremer H., Berry L., Dennis P.P. 1973; Regulation of ribonucleic acid synthesis in Escherichia colib/r: an analysis of shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates.. Journal of Molecular Biology 75:161–179
    [Google Scholar]
  4. Burgess R.R., Travers A.A., Dunn J.J., Bautz E.K.F. 1969; Factor stimulating transcription by RNA polymerase.. Nature; London: 22143–46
    [Google Scholar]
  5. Carter B.L.A., Dawes I.W. 1975; Synthesis of two DNA-dependent RNA polymerases in yeast.. Experimental Cell Research 92:253–258
    [Google Scholar]
  6. Fraser R.S.S., Creanor J., Mitchison J.M. 1973; Rapid and selective inhibition of high molecular weight RNA in yeast by lomofungin.. Nature; London: 244222–224
    [Google Scholar]
  7. Kudrna R., Edlin G. 1975; Nucleotide pools and regulation of ribonucleic acid synthesis in yeast.. Journal of Bacteriology 121:740–742
    [Google Scholar]
  8. Lacroute F., Huet J., Exinger F. 1975; Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae.. Journal of Bacteriology 122:847–854
    [Google Scholar]
  9. Nierlich D.P. 1972; Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA.. Journal of Molecular Biology 72:765–777
    [Google Scholar]
  10. Ponta H., Ponta U., Wintersberger E. 1972; Purification and properties of DNA-dependent RNA polymerase from yeast.. European Journal of Biochemistry 29:110–118
    [Google Scholar]
  11. Rosset R., Julien J., Monier R. 1966; Ribonucleic acid composition of bacteria as a function of growth rate.. Journal of Molecular Biology 18:308–320
    [Google Scholar]
  12. Schweizer E., Halvorson H.O. 1969; On the regulation of ribosomal RNA synthesis in yeast.. Experimental Cell Research 56:239–244
    [Google Scholar]
  13. Sebastian J., Mian F., Halvorson H.O. 1973; Effect of the growth rate on the level of DNA-dependent RNA polymerases in Saccharomyces cerevisiae.. FEBS Letters 34:159–162
    [Google Scholar]
  14. Skjold A.C., Juarez H., Hedgcoth C. 1973; Relationships among deoxyribonucleic acid, ribonucleic acid and specific transfer ribonucleic acids in Escherichia coli 15T at various growth rates.. Journal of Bacteriology 115:177–187
    [Google Scholar]
  15. Thonart P., Bechet J., Hilger F., Burny A. 1976; Thermosensitive mutations affecting ribonucleic acid polymerases in Saccharomyces cerevisiae.. Journal of Bacteriology 125:25–32
    [Google Scholar]
  16. Udem S.A., Kaufman K., Warner J.R. 1971; Small ribosomal ribonucleic acid species of Saccharomyces cerevisiae.. Journal of Bacteriology 105:101–106
    [Google Scholar]
  17. Valenzuela P., Hager G.L., Weinberg F., Rutter W.J. 1976; Molecular structure of yeast RNA polymerase III. Demonstration of the tripartite transcriptive system in lower eukaryotes.. Proceedings of the National Academy of Sciences of the United States of America 73:1024–1028
    [Google Scholar]
  18. Waldron C., Lacroute F. 1975; Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast.. Journal of Bacteriology 122:855–865
    [Google Scholar]
  19. Wehr C.T., Parks L.W. 1969; Macromolecular synthesis in Saccharomyces cerevisiae in different growth media.. Journal of Bacteriology 98:458–466
    [Google Scholar]
  20. Weinmann R., Roeder R.G. 1974; Role of DNA-dependent RNA polymerase III in the transcription of the tRNA and 5 S RNA genes.. Proceedings of the National Academy of Sciences of the United States of America 71:1790–1794
    [Google Scholar]
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