1887

Abstract

Summary: The influence of the DNA synthesis inhibitor, nalidixic acid, on the properties of synchronous cultures of selected swarmer cells was examined. There was little alteration in the changes in morphology, extinction, volume distribution and leucine incorporation up to bud development, and photosynthetic membrane lamellae were still synthesized in the bud. However, there was no subsequent division, or flagellum or holdfast synthesis. Instead cells elongated by continued outgrowth of the abortive bud. Since DNA synthesis was also inhibited, this suggested a dependence of cell division, and flagellum and holdfast synthesis, on the completion of chromosome replication. By addition or removal of nalidixic acid at various times in the cell cycle, periods were demonstrated when the organism was insensitive to the antibiotic indicating that there was a pre-synthetic and post-synthetic gap in the pattern of DNA synthesis in swarmers.

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/content/journal/micro/10.1099/00221287-98-1-155
1977-01-01
2021-10-18
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References

  1. Blackman M.A., Weiner R.M. 1975; Photomicrography of nalidixic acid treated Hyphomicrobium neptunium: inhibition of bud formation and bud separation.. Canadian Journal of Microbiology 21:226–230
    [Google Scholar]
  2. Bourguignon G.J., Levitt M., Sternglanz R. 1973; Studies on the mechanism of action of nalidixic acid.. Antimicrobial Agents and Chemotherapy 4:479–486
    [Google Scholar]
  3. Boyle J.V., Cook T.M., Goss W.A. 1969; Mechanism of action of nalidixic acid on Escherichia coli.VI. Cell-free studies.. Journal of Bacteriology 97:230–236
    [Google Scholar]
  4. Burdett I.D.J., Murray R.G.E. 1974; Electron microscopy study of septum formation in Escherichia coli strains b and b/r during synchronous growth.. Journal of Bacteriology 119:1039–1056
    [Google Scholar]
  5. Clark D.J. 1968; Regulation of deoxyribonucleic acid replication and cell division in Escherichia colib/r.. Journal of Bacteriology 96:1214–1224
    [Google Scholar]
  6. Cook T.M., Brown K.G., Boyle J.V., Goss W.A. 1966; Bactericidal action of nalidixic acid on Bacillus subtilis.. Journal of Bacteriology 92:1510–1514
    [Google Scholar]
  7. Degnen S.T., Newton A. 1972a; Dependence of cell division on the completion of chromosome replication in Caulobacter crescentus.. Journal of Bacteriology 110:852–856
    [Google Scholar]
  8. Degnen S.T., Newton A. 1972b; Chromosome replication during development in Caulobacter crescentus.. Journal of Molecular Biology 64:671–680
    [Google Scholar]
  9. Dix D.E., Helmstetter C.E. 1973; Coupling between chromosome replication and cell division in Escherichia coli.. Journal of Bacteriology 115:786–795
    [Google Scholar]
  10. Donachie W.D., Martin D.T.M., Begg K.J. 1971; Independence of cell division and chromosome replication in Bacillus subtilis.. Nature, New Biology 231:274–276
    [Google Scholar]
  11. Dow C.S., Westmacott D., Whittenbury R. 1976; Ultrastructure of budding and prosthecate bacteria.. In Microbial Ultrastructure.The Use of the Electron Microscope, Society of Applied Bacteriology Technical Series 10 pp. 187–220 Edited by Fuller R., Lovelock D. W. London: Academic Press;
    [Google Scholar]
  12. Goss W.A., Deitz W.H., Cook T.M. 1964; Mechanism of action of nalidixic acid on Escherichia coli.. Journal of Bacteriology 88:1112–1118
    [Google Scholar]
  13. Goss W.A., Deitz W.H., Cook T.M. 1965; Mechanism of action of nalidixic acid on Escherichia coli. II. Inhibition of deoxyribonucleic acid synthesis.. Journal of Bacteriology 89:1068–1074
    [Google Scholar]
  14. Helmstetter C. E., Pierucci O. 1968; Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli.. Journal of Bacteriology 95:1627–1633
    [Google Scholar]
  15. Javor G.T. 1974; Inhibition of ribonucleic acid synthesis by nalidixic acid in Escherichia coli.. Journal of Bacteriology 120:282–286
    [Google Scholar]
  16. Ryter A., Kellenberger E. 1958; Étude au microscope électronique de plasmas contenant de l’acide désoxyribonucléique. I. Les nucléoides des bactéries en croissance active.. Zeitschrift für Naturforschung 13b:597–605
    [Google Scholar]
  17. Sargent M.G. 1975; Control of cell length in Bacillus subtilis.. Journal of Bacteriology 123:7–19
    [Google Scholar]
  18. Shuman H., Schwartz M. 1975; The effect of nalidixic acid on the expression of some genes in Escherichia coli k-12.. Biochemical and Biophysical Research Communications 64:204–209
    [Google Scholar]
  19. Ward C.B., Hane M.W., Glaser D.A. 1970; Synchronous reinitiation of chromosome replication in E. coli b/r after nalidixic acid treatment.. Proceedings of the National Academy of Sciences of the United States of America 66:365–369
    [Google Scholar]
  20. Weiner R.M., Blackman M.A. 1973; Inhibition of deoxyribonucleic acid synthesis and bud formation by nalidixic acid in Hyphomicrobium neptunium.. Journal of Bacteriology 116:1398–1404
    [Google Scholar]
  21. Westmacott D., Primrose S.B. 1975; An anaerobic bag for photoheterotrophic growth of some Rhodospirillaceae in Petri dishes.. Journal of Applied Bacteriology 38:205–207
    [Google Scholar]
  22. Westmacott D., Primrose S.B. 1976; Synchronous growth of Rhodopseudomonas palustris from the swarmer phase.. Journal of General Microbiology 94:117–125
    [Google Scholar]
  23. Whittenbury R., Mclee A.G. 1967; Rhodopseudomonas palustris and Rh. viridis-photosynthetic budding bacteria.. Archiv für Mikrobiologie 59:324–334
    [Google Scholar]
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