1887

Abstract

SUMMARY: Solid media containing carboxymethylcellulose (CMC) were developed to detect C cellulase-producing micro-organisms. Hydrolysis of CMC was seen as a clear zone around colonies after flooding plates with 1% aqueous hexadecyltrimethyl-ammonium bromide. Tests with ten bacterial and four fungal species showed that the degree of substitution (DS) of the CMC affects both growth and enzyme production. Most of the organisms produced more C cellulase on CMC with a DS of 0·9, but CMC with a DS of 0·4 was better for one fungus. A qualitative measure of cellulase production may be obtained by calculating the ratio of zone size to colony diameter. Solid media containing CMC provided a more rapid assay of C cellulase production than a medium containing native cellulose.

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/content/journal/micro/10.1099/00221287-98-1-109
1977-01-01
2021-05-10
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