Summary: Fluorescent antibodies (FA) prepared against the Mo-Fe and Fe proteins of nitrogenase from 5a1 were used to detect these protein components in toluene-treated whole cells that were actively reducing acetylene. The FA were highly specific, staining only nitrogenase component proteins originating from Klebsiella. Cross-reactions between the FA and purified nitrogenase proteins from other dinitrogen-fixing micro-organisms did not occur, except in the case of . The tests rapidly and accurately assayed the component proteins in Klebsiella mutants and derivatives to which Klebsiella genes had been transferred either by plasmid or by other means. Cross-reactions also indicated the degree of relatedness between nitrogenase proteins from dinitrogen-fixing micro-organisms of various origins.


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