Rhodanese from Thiobacillusa2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

MARVIN SILVER; O.W. HOWARTH; D.P. KELLY

doi:10.1099/00221287-97-2-285

Rhodanese from Thiobacillus a2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

MARVIN SILVER, O.W. HOWARTH and D.P. KELLY
First Published: 01 December 1976 https://doi.org/10.1099/00221287-97-2-285

Abstract

Summary: Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of α-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry.

Received: 12/04/1976
Published Online: 01/12/1976

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Microbiology

Volume 97, Issue 2

Research Article

Free

Rhodanese from Thiobacillus a2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

Abstract

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