Rhodanese from Thiobacillusa2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

MARVIN SILVER; O.W. HOWARTH; D.P. KELLY

doi:10.1099/00221287-97-2-285

Rhodanese from Thiobacillus a2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

MARVIN SILVER, O.W. HOWARTH and D.P. KELLY
First Published: 01 December 1976 https://doi.org/10.1099/00221287-97-2-285

Abstract

Summary: Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of α-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry.

Received: 12/04/1976
Published Online: 01/12/1976

Article metrics loading...

/content/journal/micro/10.1099/00221287-97-2-285

1976-12-01

2021-03-06

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-97-2-285

Most cited this month Most Cited RSS feed

- Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
  
  Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman and Roger Y. Stanier
- Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin
  
  Alexander Bolotin, Benoit Quinquis, Alexei Sorokin and S. Dusko Ehrlich
- Metals, minerals and microbes: geomicrobiology and bioremediation
  
  Geoffrey Michael Gadd
- Plant-beneficial effects of Trichoderma and of its genes
  
  Rosa Hermosa, Ada Viterbo, Ilan Chet and Enrique Monte
- Short motif sequences determine the targets of the prokaryotic CRISPR defence system
  
  F. J. M. Mojica, C. Díez-Villaseñor, J. García-Martínez and C. Almendros
- Preparation of Cell-free Bacterial Extracts with Powdered Alumina
  
  H. Mcilwain
- The Aerobic Pseudomonads a Taxonomic Study
  
  R. Y. STANIER, N. J. PALLERONI and M. DOUDOROFF
- Quantification of biofilm structures by the novel computer program comstat
  
  Arne Heydorn, Alex Toftgaard Nielsen, Morten Hentzer, Claus Sternberg, Michael Givskov, Bjarne Kjær Ersbøll and Søren Molin
- CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies
  
  C. Pourcel, G. Salvignol and G. Vergnaud
More Less

Microbiology

Volume 97, Issue 2

Research Article

Free

Rhodanese from Thiobacillus a2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy

Abstract

Most read this month

Most cited this month Most Cited RSS feed

Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria

Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin

Metals, minerals and microbes: geomicrobiology and bioremediation

Plant-beneficial effects of Trichoderma and of its genes

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

Preparation of Cell-free Bacterial Extracts with Powdered Alumina

The Aerobic Pseudomonads a Taxonomic Study

Quantification of biofilm structures by the novel computer program comstat

CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies