1887

Abstract

Summary: Synchronous cultures of yeast and hyphal phases of showed exponential increases in RNA content and stepwise exponential increases in DNA content. The periods of DNA synthesis in the two phases coincided with one another and with the budding peaks of the yeast phase. Hyphae grown in synchronous cultures also showed an exponential increase in length. The hyphal phase was therefore normal.

Hyphal nuclear division occurred after hyphal DNA synthesis. Germination was a unique event for a hypha and unlike yeast bud formation, preceded the first period of DNA synthesis.

The exponential increase in RNA and DNA in asynchronous cultures of hyphae in serum paralleled the exponential increase in the numbers of cells in asynchronous cultures of yeasts in defined media. Thus there are no factors in serum which inhibit the normal exponential growth of .

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/content/journal/micro/10.1099/00221287-97-2-211
1976-12-01
2021-05-15
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References

  1. Bainbridge B.W. 1971; Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. Journal of General Microbiology 66:319–325
    [Google Scholar]
  2. Bostock C.J. 1970; DNA synthesis in the fission yeast Schizosaccharomyces pombe. Experimental Cell Research 60:16–26
    [Google Scholar]
  3. Dastidar S.G. 1965; Studies on nucleic acids and protein contents of Candida albicans and their relationship to dimorphism. Indian Journal of Experimental Biology 4:51–52
    [Google Scholar]
  4. Davies R.R., Denning T.J.V. 1972; Growth and form in Candida albicans. Sabouraudia 10:180–188
    [Google Scholar]
  5. Davies R.R., Savage M.A. 1974; Observations on 5-fluorocytosine and Candida albicans. Sabouraudia 12:302–308
    [Google Scholar]
  6. Elin R.J., Wolff S.M. 1973; Effect of pH and iron concentration on growth of Candida albicans in human serum. Journal of Infectious Diseases 127:705–708
    [Google Scholar]
  7. Evans E.G.V., Odds F.C., Holland K.T. 1975; Resistance of the Candida albicans filamentous cycle to environmental change. Sabouraudia 13:231–238
    [Google Scholar]
  8. Hendry A.T. 1972; Inhibition of catalase activity of Candida albicans by serum. Sabouraudia 10:193–204
    [Google Scholar]
  9. Mitchison J.M. 1970 Methods in Cell Physiology, 4 p. 131 Prescott. D.M. Edited by New York: Academic Press.;
    [Google Scholar]
  10. Mitchison J.M. 1971 The Biology of the Cell Cycle. Cambridge: Cambridge University Press.;
    [Google Scholar]
  11. Mitchison J.M., Vincent W.S. 1965; Preparation of synchronous cell cultures by sedimentation. Nature; London: 205987–989
    [Google Scholar]
  12. Partridge B.M., Athar M.A., Winner H.I. 1971; Chick embryo inoculation as a pathogenicity test for Candida species. Journal of Clinical Pathology 24:645–648
    [Google Scholar]
  13. Poole R.K., Lloyd D., Kemp R.B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h. Journal of General Microbiology 77:209–220
    [Google Scholar]
  14. Trinci A.P.J. 1974; A study of the kinetics of hyphal extension and branch initiation of fungal mycelia. Journal of General Microbiology 81:225–236
    [Google Scholar]
  15. Wain W.H., Staatz W.D. 1973; Rates of synthesis of ribosomal protein and total ribonucleic acid through the cell cycle of the fission yeast Schizosaccharomyces pombe. Experimental Cell Research 81:264–278
    [Google Scholar]
  16. Wain W.H., Price M.F., Cawson R.A. 1974; The fate of Candida albicans inoculated on to the chick chorio-allantoic membrane. Journal of Dental Research 53:1066
    [Google Scholar]
  17. Wain W.H., Price M.F., Cawson R.A. 1975; A re-evaluation of the effect of cysteine on Candida albicans. Sabouraudia 13:74–82
    [Google Scholar]
  18. Williamson D.H., Scopes A.W. 1962; A rapid method for synchronizing division in the yeast Saccharomyces cerevisiae. Nature; London: 193256–257
    [Google Scholar]
  19. Winner H.I., Hurley R. 1964 Candida albicans p. 33 London: Churchill.;
    [Google Scholar]
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