1887

Abstract

Summary: Mitosis, septation and branching were studied in undifferentiated mycelia and leading hyphae of , a mould which forms incomplete septa. After spore germination, nuclei divided synchronously until germ-tube hyphae contained 8 or 16 nuclei; mitosis occurred when the volume of cytoplasm per haploid nucleus was about 60 m. Intercompartment development was not synchronized, consequently mitosis in the mycelium as a whole eventually became asynchronous. During the stage of asynchronous compartment development, the nuclei, septa, branches and total length of undifferentiated mycelia all increased exponentially at approximately the same specific rate.

Septa were formed in hyphae in groups of up to nine; the mean time required for the formation of a group of septa was about 9 min. The mean interval between successive cycles of septation in a hypha was approximately the same as the organism's doubling time. There was a highly significant correlation coefficient between septation and branch initiation and most intercalary compartments initially formed a single branch.

The volume of cytoplasm per nucleus in a diploid strain was approximately double the value observed for a haploid strain. However, the length of the hyphal growth unit was not affected by ploidy.

The study suggests that a duplication cycle can be recognized during mycelial growth which is analogous to the cell cycle observed in unicellular micro-organisms.

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1976-12-01
2024-04-24
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References

  1. Bainbridge B.W. 1976; Estimation of the generation time and peripheral growth zone of Aspergillus nidulans and Alternaria solani hyphae from radial growth rates and ranges of apical cell length. Journal of General Microbiology 97:125–127
    [Google Scholar]
  2. Bull A.T., Trinci A.P.J. 1976; The physiology and metabolic control of fungal growth. Advances in Microbial Physiology in the Press
    [Google Scholar]
  3. Campbell A. 1957; Synchronization of cell division. Bacteriological Reviews 21:253–272
    [Google Scholar]
  4. Clutterbuck A.J. 1969; Cell volume per nucleus in haploid and diploid strains of Aspergillus nidulans. Journal of General Microbiology 55:291–300
    [Google Scholar]
  5. Clutterbuck A.J. 1970; Synchronous nuclear division and septation in Aspergillus nidulans. Journal of General Microbiology 60:133–135
    [Google Scholar]
  6. Clutterbuck A.J., Roper J.A. 1966; A direct determination of nuclear distribution in heterokaryons of Aspergillus nidulans. Genetical Research, Cambridge 7:185–194
    [Google Scholar]
  7. Cole O.T., Kendrick W.B. 1968; A thin culture chamber for time lapse photomicrography of fungi at high magnifications. Mycologia 60:340–342
    [Google Scholar]
  8. Collinge A.J., Trinci A.P.J. 1974; Hyphal tips of wild-type and spreading colonial mutants of Neurospora crassa. Archives of Microbiology 99:353–368
    [Google Scholar]
  9. Fencl Z., Machek F., Novak M. 1969; Kinetics of product formation in multistage continuous culture. In Fermentation Advances p. 301 Perlman D. Edited by New York and London: Academic Press.;
    [Google Scholar]
  10. Fiddy C., Trinci A.P.J. 1976; Nuclei, septation, branching and growth of Geotrichum candidum. Journal of General Microbiology 97:185–192
    [Google Scholar]
  11. Gooday G.W. 1971; An autoradiographic study of hyphal growth of some fungi. Journal of General Microbiology 67:125–133
    [Google Scholar]
  12. Grove S.W., Bracker C.E. 1970; Protoplasmic organization of hyphal tips among fungi: vesicles and spitzenkörper. Journal of Bacteriology 104:989–1009
    [Google Scholar]
  13. Johnson B.J., Yoo B.Y., Calleja G.B. 1973; Cell division in yeasts: movement of organelles associated with cell plate growth of Schizosaccharomyces pombe. Journal of Bacteriology 115:358–366
    [Google Scholar]
  14. King S.B., Alexander L.J. 1969; Nuclear behaviour, septation and hyphal growth of Alternaria solani. American Journal of Botany 56:249–253
    [Google Scholar]
  15. Lhoas P. 1968; Growth rate haploidization of Aspergillus niger in medium containing p-fluorophenyl-alanine. Genetical Research, Cambridge 12:305–315
    [Google Scholar]
  16. Mitchison J.M. 1971 The Biology of the Cell Cycle. Cambridge: Cambridge University Press.;
    [Google Scholar]
  17. Morris N.R. 1975; Mitotic mutants of Aspergillus nidulans. Genetical Research, Cambridge 26:237–254
    [Google Scholar]
  18. Niederpruem D.J., Jersild R.A. 1972; Cellular aspects of morphogenesis in the mushroom Schizophyllum commune. Critical Reviews in Microbiology545–576
    [Google Scholar]
  19. Nishi A., Yanagita T., Maruyama Y. 1968; Cellular events occurring in growing hyphae of Aspergillus oryzae as studied by autoradiography. Journal of General and Applied Microbiology 14:171–182
    [Google Scholar]
  20. Robinow C.F. 1963; Observations on cell growth, mitosis, and division in the fungus Basidiobolus ranarum. Journal of Cell Biology 17:123–152
    [Google Scholar]
  21. Rosenberger R.F., Kessel M. 1967; Synchrony of nuclear replication in individual hyphae of Aspergillus nidulans. Journal of Bacteriology 94:1464–1469
    [Google Scholar]
  22. Sachsenmaier W., Remy V., Plattner-Shobel R. 1972; Initiation of synchronous mitosis in Physarum polycephalum. A model of the control of cell division in eukaryotes. Experimental Cell Research 73:41–48
    [Google Scholar]
  23. Steele G.C., Trinci A.P.J. 1975; Morphology and growth kinetics of hyphae of differentiated and undifferentiated mycelia of Netarospora crassa. Journal of General Microbiology 91:362–368
    [Google Scholar]
  24. Trinci A.P.J. 1971; Influence of the width of the peripheral growth zone on the radial growth rate of fungal colonies on solid media. Journal of General Microbiology 67:325–344
    [Google Scholar]
  25. Trinci A.P.J. 1973; The hyphal growth unit of wild-type and spreading colonial mutants of Neurospora crassa. Archiv für Mikrobiologie 91:127–136
    [Google Scholar]
  26. Trinci A.P.J. 1974; A study of the kinetics of hyphal extension and branch initiation of fungal mycelia. Journal of General Microbiology 81:225–236
    [Google Scholar]
  27. Trinci A.P.J., Collinge A.J. 1973; Structure and plugging of septa of wild-type and spreading colonial mutants of Neurospora crassa. Archiv für Mikrobiologie 91:355–364
    [Google Scholar]
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