1887

Abstract

SUMMARY: The linkage of two mutations leading to increased sensitivity to ultraviolet light and Co gamma rays was determined in the slime mould using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids on the basis of simultaneous radiation and temperature resistance. Analysis of drug-resistant haploid segregants of the heterozygous diploids indicated that one of the radiation-sensitive mutations, , was linked to linkage group 1 whereas the other, , was linked to the recently defined linkage group VI.

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1976-11-01
2021-05-08
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References

  1. Brody T., Williams K. L. 1974; Cytological analysis of the parasexual cycle inDictyostelium dis- coideum. Journal of General Microbiology 82:371–383
    [Google Scholar]
  2. Deering R. A. 1968 Dictyostelium discoideum: a gamma-ray resistant organism. Science; New York: 162:1289–1290
    [Google Scholar]
  3. Deering R. A. 1975; Dictyostelium discoideum: a valuable eukaryotic system for repair studies. In Molecular Mechanisms for Repair of DNA part B pp. 581–584 New York: Plenum Publishing Corp.;
    [Google Scholar]
  4. Deering R. A., Jensen D. S. 1973; Nuclear and mitochondrial DNA synthesis in gamma ray-resistant and -sensitive slime mold amoebas. BiophysicalJournal 13(2):780–794
    [Google Scholar]
  5. Deering R. A., Jensen D. S. 1975; Endonuclease fromDictyostelium discoideum that attacks ultraviolet-irradiated deoxyribonucleic acid. Journal of Bacteriology 121:1211–1213
    [Google Scholar]
  6. Deering R. A., Smith M. S., Thompson B. K., Adolf A. C. 1970; Gamma-ray-resistant and -sensitive strains of slime mold{Dictyostelium discoideum). Radiation Research 43:711–728
    [Google Scholar]
  7. Free S. J., Loomis W. F. 1974; Isolation of mutations inDictyostelium discoideum affecting α-mannosidase. Biochimie 56:1525–1528
    [Google Scholar]
  8. Free S. J., Schimke R. T., Loomis W. F. 1976; The structural gene for α-mannosidase-i inDictyostelium discoideum. Genetics in the Press
    [Google Scholar]
  9. Freim J. O., Deering R. A. 1970; Ultraviolet irradiation of the vegetative cells ofDictyostelium discoideum. Journal of Bacteriology 102:36–42
    [Google Scholar]
  10. Gingold E. B., Ashworth J. M. 1974; Evidence for mitotic crossing-over during the parasexual cycle of the cellular slime mouldDictyostelium discoideum. Journal of General Microbiology 84:70–78
    [Google Scholar]
  11. Guialis A., Deering R. A. 1976a; Repair of deoxyribonucleic acid in ultraviolet light-sensitive and -resistantDictyostelium discoideum strains. Journal of Bacteriology 127:59–66
    [Google Scholar]
  12. Guialis A., Deering R. A. 1976b; Inhibition of UV-induced DNA strand breakage inDictyostelium discoideum by 2,4-dinitrophenol. Photochemistry and Photobiology in the Press
    [Google Scholar]
  13. Jagger J. 1961; A small and inexpensive ultraviolet dose-rate meter useful in biological experiments. Radiation Research 14:394–403
    [Google Scholar]
  14. Katz E. R., Kao V. 1974; Evidence for mitotic recombination in the cellular slime moldDictyostelium discoideum. Proceedings of the National Academy of Sciences of the United States of America 71:4025–4026
    [Google Scholar]
  15. Katz E. R., Sussman M. 1972; Parasexual recombination inDictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants. Proceedings of the National Academy of Sciences of the United States of America 69:495–498
    [Google Scholar]
  16. Khoury A. T., Deering R. A. 1973; Sedimentation of DNA ofDictyostelium discoideum lysed on alkaline sucrose gradients: role of single-strand breaks in gamma ray lethality of sensitive and resistant strains. Journal of Molecular Biology 79:267–284
    [Google Scholar]
  17. Loomis W. F. 1969; Temperature-sensitive mutants ofDictyostelium discoideum. Journal of Bacteriology 99:65–69
    [Google Scholar]
  18. Loomis W. F. 1970; Developmental regulation of α-mannosidase inDictyostelium discoideum. Journal of Bacteriology 103:375–381
    [Google Scholar]
  19. Morowitz H. J. 1950; Absorption effects in volume irradiations of microorganisms. Science; New York: 111:229–230
    [Google Scholar]
  20. Mosses D., Williams K. L., Newell P. C. 1975; The use of mitotic crossing-over for genetic analysis inDictyostelium discoideum: mapping of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  21. Payez J. F., Deering R. A. 1972; Synergistic and antagonistic effects of caffeine on two strains of cellular slime mold treated with alkylating agents. Mutation Research 16:318–321
    [Google Scholar]
  22. Payez J. F., Deering R. A., Freim J. O. 1972; Lethal effects of alkylating agents or ultraviolet light on the gamma-ray resistant parent strain and a gamma-ray sensitive strain of the cellular slime moldDictyostelium discoideum. Mutation Research 15:82–85
    [Google Scholar]
  23. Pontecorvo G., Käfer E. 1958; Genetic analysis based on mitotic recombination. Advances in Genetics 9:71–104
    [Google Scholar]
  24. Sackin M. J., Ashworth J. M. 1969; An analysis of the distribution of volumes amongst spores of the cellular slime mouldDictyostelium discoideum. Journal of General Microbiology 59:275–284
    [Google Scholar]
  25. Sinha U., Ashworth J. M. 1969; Evidence for the existence of elements of a parasexual cycle in the cellular slime mould, Dictyostelium discoideum. Proceedings of the Royal Society B173:531–540
    [Google Scholar]
  26. Williams K. L., Kessin R. H., Newell P. C. 1974a; Parasexual genetics inDictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
  27. Williams K. L., Kessin R. H., Newell P. C. 1974b; Genetics of growth in axenic medium of the cellular slime mouldDictyostelium discoideum. Nature; London: 247:142–143
    [Google Scholar]
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