SUMMARY: Lipopolysaccharide (LPS) of was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage inactivation (PI = 0·05 μg LPS ml), whereas LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (pH 7·4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to 1 mg ml, but produced weak chlorosis in bean and tobacco at 2mg ml: no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined.


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