Regulation of Growth of 8250 on Benzyl Alcohol in Batch Culture Free

Abstract

SUMMARY: Formation of benzoate and catechol during oxidation of benzyl alcohol by washed suspensions of 8250 confirmed earlier results indicating that this organism metabolizes benzyl alcohol via benzaldehyde, benzoate, and the 3-oxoadipate pathway. There was no evidence for feedback inhibition of benzyl alcohol dehydrogenase or benzaldehyde dehydrogenase II. Examination of growth curves and patterns of substrate utilization, as well as measurement of enzyme activities, showed that benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II are repressed when utilizes -mandelate or phenylglyoxylate. Growth of bacteria on -mandelate prior to their inoculation into benzyl alcohol/salts medium leads to an exceptionally long lag period before benzyl alcohol is used at the maximum rate. Benzyl alcohol metabolism is also suppressed during growth on benzoate.

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/content/journal/micro/10.1099/00221287-96-2-365
1976-10-01
2024-03-28
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References

  1. Arnow L. E. 1937; Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. Journal of Biological Chemistry 118:531–537
    [Google Scholar]
  2. Beggs J. D. 1974 Control of the metabolism of 1-mandelate and benzyl alcohol in Acinetobactercalcoaceticus n.c.i.b. 8250. Ph.D. thesis University of Glasgow.:
    [Google Scholar]
  3. Beggs J. D., Fewson C. A. 1974; Repression of the enzymes converting benzyl alcohol into benzoate in Acinetobacter calcoaceticus n.c.i.b. 8250. Biochemical Society Transactions 2:924–925
    [Google Scholar]
  4. Cock A. M., Fewson C. A. 1972a; Evidence for specific transport mechanisms for aromatic compounds in bacterium n.c.i.b. 8250. Biochimica et biophysica acta 290:384–388
    [Google Scholar]
  5. Cook A. M., Fewson C. A. 1972b; Utilisation of pairs of substrates by bacterium n.c.i.b. 8250 growing in batch culture. Biochemical Journal 127:77–78P
    [Google Scholar]
  6. Cook A. M., Beggs J. D., Fewson C. A. 1975; Regulation of growth of Acinetobacter calcoaceticus ncib8250 on l-mandelate in batch culture. Journal of General Microbiology 91:325–337
    [Google Scholar]
  7. Fewson C. A. 1966; Alcohol dehydrogenase activity of organism n.c.i.b. 8250. Biochemical Journal 101:21P
    [Google Scholar]
  8. Fewster M. E., Hall D. A. 1951; Application of buffered solvent systems to the detection of aromatic acids by paper partition chromatography. Nature; London: 16878–79
    [Google Scholar]
  9. Gibson D. T. 1971; The microbial oxidation of aromatic hydrocarbons. Critical Reviews in Microbiology 1:199–223
    [Google Scholar]
  10. Goldman P., Milne G.W.A., Pignataro M. T. 1967; Fluorine containing metabolites formed from 2-fluorobenzoic acid by Pseudomonas species. Archives of Biochemistry and Biophysics 118:178–184
    [Google Scholar]
  11. Hamilton W. A., Dawes E. A. 1959; A diauxic effect with Pseudomonas aeruginosa. Biochemical Journal 71:25–26P
    [Google Scholar]
  12. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. Journal of Bacteriology 91:1140–1154
    [Google Scholar]
  13. Higgins S. J., Mandelstam J. 1972; Regulation of pathways degrading aromatic substrates in Pseudomonas putida. Enzymic response to binary mixture of substrates. Biochemical Journal 126:901–916
    [Google Scholar]
  14. Holms W. H., Robertson A. G. 1974; Control of derepressed β-galactosidase synthesis in Escherichia coli. Archives of Microbiology 96:21–35
    [Google Scholar]
  15. Kennedy S.I.T., Fewson C. A. 1968a; Metabolism of mandelate and related compounds by bacterium NCIB8250. Journal of General Microbiology 53:259–273
    [Google Scholar]
  16. Kennedy S.I.T., Fewson C. A. 1968b; Enzymes of the mandelate pathway in bacterium n.c.i.b. 8250. Biochemical Journal 107:497–506
    [Google Scholar]
  17. Livingstone A., Fewson C. A. 1972; Regulation of the enzymes converting l-mandelate into benzoate in bacterium n.c.i.b. 8250. Biochemical Journal 130:937–946
    [Google Scholar]
  18. Livingstone A., Fewson C. A., Kennedy S.I.T., Zatman L. J. 1972; Two benzaldehyde dehydrogenases in bacterium n.c.i.b. 8250. Distinguishing properties and regulation. Biochemical Journal 130:927–935
    [Google Scholar]
  19. Murphy G., Lynen F. 1975; Patulin biosynthesis: the metabolism of m-hydroxybenzyl alcohol and m-hydroxybenzaldehyde by particulate preparations from Penicillium patulum. European Journal of Biochemistry 58:467–475
    [Google Scholar]
  20. Nozaka J., Kusunose M. 1968; Metabolism of hydrocarbons in microorganisms. Part I. Oxidation of p-xylene and toluene by cell-free enzyme preparations of Pseudomonas aeruginosa. Agricultural and Biological Chemistry 32:1033–1039
    [Google Scholar]
  21. Stevenson I. L., Mandelstam J. 1965; Induction and multi-sensitive end-product repression in two converging pathways degrading aromatic substances in Pseudomonas fluorescens. Biochemical Journal 96:354–362
    [Google Scholar]
  22. Wilson D. W. 1947 A Laboratory Manual of Physiological Chemistry, 6th edn. p. 232 Baltimore:: Williams & Wilkins.;
    [Google Scholar]
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