1887

Abstract

SUMMARY: -Aminocaproic acid (EACA) inhibited the development of strain 2 after the aggregation stage. Biochemical changes that occurred early in development (loss of cellular protein, RNA and carbohydrate; increase in the specific activity of --acetylglucosaminidase, a-mannosidase, threonine deaminase and leucine aminopeptidase) were not affected by concentrations of EACA which blocked development; but biochemical changes that occurred later (synthesis of carbohydrate, increase in the specific activity of UDP-glucose pyrophosphorylase) were inhibited. Spores from fruiting bodies formed in the presence of low concentrations of EACA were larger, more spherical and less able to survive heat treatment than spores from fruiting bodies of control (no EACA) cells.

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1976-09-01
2024-12-04
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References

  1. Ablondi F. B., Hagan J. J., Phillips M., De Renzo E. C. 1959; Inhibition of plasmin, trypsin and the streptokinase-activated fibrinolytic system by ɛ-aminocaproic acid. Archives of Biochemistry and Biophysics 82:153–160
    [Google Scholar]
  2. Brackenbury R., Sussman M. 1975; Mutant of Dictyostelium discoideum defective in cell contact regulation of enzyme expression. Cell 4:347–352
    [Google Scholar]
  3. Cotter D. A., Raper K. B. 1966; Spore germination in Dictyostelium discoideum. Proceedings of the National Academy of Sciences of the United States of America 56:880–887
    [Google Scholar]
  4. Donner L., Housková J. 1967; On the action mechanism of some fibrinolysis inhibitors. Thrombosis et diathesis haemorrhagica 18:439–446
    [Google Scholar]
  5. Edmundson T. D., Ashworth J. M. 1972; 6-Phosphogluconate dehydrogenase and the assay of uridine diphosphate glucose pyrophosphorylase in the cellular slime mould Dictyostelium discoideum. Biochemical Journal 126:593–600
    [Google Scholar]
  6. Firtel R. A., Brackenbury R. W. 1972; Partial characterization of several protein and amino acid metabolizing enzymes in the cellular slime mould Dictyostelium discoideum. Developmental Biology 27:307–321
    [Google Scholar]
  7. Franke J., Sussman M. 1973; Accumulation of uridine diphosphoglucose pyrophosphorylase in Dictyostelium discoideum via preferential synthesis. Journal of Molecular Biology 81:173–185
    [Google Scholar]
  8. Garrod D. R., Ashworth J. M. 1972; Effect of growth conditions on development of the cellular slime mould, Dictyostelium discoideum. Journal of Embryology and Experimental Morphology 28:463–479
    [Google Scholar]
  9. Gustafson G. L., Kong W. Y., Wright B. E. 1973; Analysis of uridine diphosphate-glucose pyrophosphorylase synthesis during differentiation in Dictyostelium discoideum. Journal of Biological Chemistry 248:5188–5196
    [Google Scholar]
  10. Hames B. D. 1972 The metabolic control of development in the cellular slime mould Dictyostelium discoideum. Ph.D. thesis University of Leicester:
    [Google Scholar]
  11. Hames B. D., Ashworth J. M. 1974a; The metabolism of macromolecules during the differentiation of myxamoebae of the cellular slime mould Dictyostelium discoideum containing different amounts of glycogen. Biochemical Journal 142:301–315
    [Google Scholar]
  12. Hames B. D., Ashworth J. M. 1974b; The control of saccharide synthesis during development of myxamoebae of Dictyostelium discoideum containing differing amounts of glycogen. Biochemical Journal 142:317–325
    [Google Scholar]
  13. Hames B. D., Weeks G., Ashworth J. M. 1972; Glycogen synthetase and the control of glycogen synthesis in the cellular slime mould Dictyostelium discoideum during cell differentiation. Biochemical Journal 126:627–633
    [Google Scholar]
  14. Hassid W. Z., Abraham S. 1957; Chemical procedures for analysis of polysaccharides. In Methods in Enzymology 3 pp. 34–50 Colowick S. P., Kaplan N. O. Edited by New York and London: Academic Press;
    [Google Scholar]
  15. Loomis W. F. 1969; Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum. Journal of Bacteriology 97:1149–1154
    [Google Scholar]
  16. Loomis W. F. 1970; Developmental regulation of α-mannosidase in Dictyostelium discoideum. Journal of Bacteriology 103:375–381
    [Google Scholar]
  17. Loomis W. F. 1975 Dictostelium discoideum: a Developmental System. New York: Academic Press;
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Mejbaum W. 1939; Über die Bestimmung kleiner Pentosemengen imbesondere in Derivaten der Adenyl- säure. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 258:117–120
    [Google Scholar]
  20. Newell P. C., Sussman M. 1969; Uridine diphosphate glucose pyrophosphorylase in Dictyostelium discoideum. Journal of Biological Chemistry 244:2990–2995
    [Google Scholar]
  21. North M. J., Ashworth J. M. 1976; Inhibition of the development of the cellular slime mould Dictyostelium discoideum by ω-aminocarboxylic acids. Journal of General Microbiology 96:63–75
    [Google Scholar]
  22. Pong S. S., Loomis W. F. 1973; Replacement of an anabolic threonine deaminase by a catabolic threonine deaminase during development of Dictyostelium discoideum. Journal of Biological Chemistry 248:4867–4873
    [Google Scholar]
  23. Quance J., Ashworth J. M. 1972; Enzyme synthesis in the cellular slime mould Dictyostelium discoideum during the differentiation of myxamoebae grown axenically. Biochemical Journal 126:609–615
    [Google Scholar]
  24. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mould development. In Methods in Cell Physiology 2 pp. 397–410 Prescott D. M. Edited by New York: Academic Press;
    [Google Scholar]
  25. Watts D. J., Ashworth J. M. 1970; Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
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